Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30750
Gaunt SJ, et al., Spatially restricted domains of homeo-gene transcripts in mouse embryos: relation to a segmented body plan. Development. 1988;104(Suppl):169-79
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1344202
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Gene symbol:
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Hoxa5
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Gene name:
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homeobox A5
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Probe:
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Hoxa5 probe2
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Probe preparation:
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Antisense labelled with S35 RNA
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Results
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Specimen
3B:
embryonic day 12.5
(more  )
Specimen
3B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thyroid gland |
Present |
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| TS20: pharynx |
Absent |
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Expression was similar to that described for Hox-1.4 (see assay MGI:1344203): Transcripts were generally not present in tissues that formed the floor of the pharynx. Transcripts were not detectable anterior to the thyroid duct.
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| TS20: trachea |
Present |
Regionally restricted |
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Sections were labelled within mesodermal but not endodermal (lining epithelial) components.
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| TS20: lung |
Present |
Regionally restricted |
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Labelling was restricted to mesodermal components and did not include the endodermally derived lung epithelium.
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| TS20: outflow tract aortic component |
Weak |
Regionally restricted |
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Transcripts were detected within the walls of the aortic trunk.
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| TS20: spinal cord |
Present |
Graded |
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A progressive anterior-to-posterior fall in abundance of transcripts was observed.
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Specimen
Table 1:
embryonic day 12.5
(more )
Specimen
Table 1:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
6C:
embryonic day 12.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: central nervous system |
Present |
Regionally restricted |
6C
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The anterior boundary lay in the posterior floor of the myelencephalon. The bounday is irregular in shape along the dorsoventral axis of the nervous tissue. These irregularities have been found to be consistent among different embryos.
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| TS20: medulla oblongata floor plate |
Present |
Regionally restricted |
6C
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The anterior boundary lay in the posterior floor of the myelencephalon. The bounday is irregular in shape along the dorsoventral axis of the nervous tissue. These irregularities have been found to be consistent among different embryos.
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Analysis Tools