Probe:	Hox-2.3 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The postimplantation development of the Swiss mice lags up to half a day behind that described in Theiler.

Structure	Level	Pattern	Image	Note
TS11: allantois	Present		1A/B
TS11: embryo	Absent		1A/B

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1C/D	Hybridization was found in the neurectoderm and presomitic mesoderm in the posterior half of the embryo.
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	1C/D	Hybridization was found in the neurectoderm and presomitic mesoderm in the posterior half of the embryo.
TS13: primitive streak	Present		1C/D
TS13: hindgut diverticulum	Present	Regionally restricted	1C/D	Hybridization was found in the hindgut epithelium and mesenchyme.
TS13: foregut diverticulum	Absent		1C/D
TS13: head	Absent		1C/D
TS13: head somite	Absent		1C/D
TS13: heart	Absent		1C/D
TS13: extraembryonic component	Absent		1C/D	No expression was detected in the amnion.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	2A/B	The domain of Hox-2.3 expression started in the upper spinal cord and extended to the caudal end of the embryo.
TS15: trunk mesenchyme	Present	Regionally restricted	2A/B	Expression was detected in the unsegmented or lateral plate mesoderm within the AP domain of expression defined in the somitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	2C/D	The domain of Hox-2.3 expression started in the upper spinal cord and extended to the caudal end of the embryo.
TS15: trunk mesenchyme	Present	Regionally restricted	2C/D	Expression was detected in the unsegmented or lateral plate mesoderm within the AP domain of expression defined in the somitic mesoderm.

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted	2E/F	The rostral boundary of expression in the mesoderm was found more caudal than that in the CNS. The anterior boundary in the somitic mesoderm was located around somite 11 and expression extended to the caudal end of the embryo.
TS15: trunk somite	Strong		2E/F	The expression level in the somites was higher than in the neural tube.
TS15: neural tube	Moderate		2E/F	The expression level in the somites was higher than in the neural tube.
TS15: forelimb bud mesenchyme	Present	Graded	2E/F	The maximal concentration of transcripts was located in the posterior half of the limb buds.

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present	Regionally restricted	2G/H	The rostral boundary of expression in the mesoderm was found more caudal than that in the CNS. The anterior boundary in the somitic mesoderm was located around somite 11 and expression extended to the caudal end of the embryo.
TS15: trunk somite	Strong		2G/H	The expression level in the somites was higher than in the neural tube.
TS15: neural tube	Moderate		2G/H	The expression level in the somites was higher than in the neural tube.
TS15: forelimb bud mesenchyme	Present	Graded	2G/H	The maximal concentration of transcripts was located in the posterior half of the limb buds.

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present	Regionally restricted	3A/B	The domain of Hox-2.3 expression started in the upper spinal cord and extended to the caudal end of the embryo.
TS16: spinal ganglion	Strong		3A/B
TS16: embryo mesenchyme	Present	Regionally restricted	3A/B	Expression could be detected in the somitic mesoderm.
TS16: embryo	Present	Single cells	3A/B	Groups of labelled cells (possibly part of the alimentary system) located between the dorsal aorta and the foregut at the level of the forelimb bud were found one or two somites more anteriorly than the most rostrally expressing somite.
TS16: forelimb bud mesenchyme	Present	Graded	3A/B	Expression was more prominent in the posterior and dorsal part of the forelimb bud. In the anterior part, expression was restricted to dorsally and distally located cells, while more posteriorly, the boundary was located progressively more ventrally.
TS16: mesonephric tubule	Strong		3A/B
TS16: nephric duct	Strong		3A/B

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present	Regionally restricted	3C/D	The domain of Hox-2.3 expression started in the upper spinal cord and extended to the caudal end of the embryo.
TS16: spinal ganglion	Strong		3C/D
TS16: embryo mesenchyme	Present	Regionally restricted	3C/D	Expression could be detected in the somitic mesoderm.
TS16: embryo	Present	Single cells	3C/D	Groups of labelled cells (possibly part of the alimentary system) located between the dorsal aorta and the foregut at the level of the forelimb bud were found one or two somites more anteriorly than the most rostrally expressing somite.
TS16: gut	Present	Regionally restricted	3C/D	Transcripts were found in the mesoderm surrounding the midgut.
TS16: hindgut epithelium	Present		3C/D
TS16: hindgut mesenchyme	Present		3C/D
TS16: hindgut	Present	Regionally restricted	3C/D	The tailgut mesenchyme and epithelium were labelled.
TS16: forelimb bud mesenchyme	Present	Graded	3C/D	Expression was more prominent in the posterior and dorsal part of the forelimb bud. In the anterior part, expression was restricted to dorsally and distally located cells, while more posteriorly, the boundary was located progressively more ventrally.
TS16: forelimb bud ectoderm	Absent		3C/D
TS16: mesonephric tubule	Strong		3C/D
TS16: nephric duct	Strong		3C/D

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present	Regionally restricted	3E/F	The domain of Hox-2.3 expression started in the upper spinal cord and extended to the caudal end of the embryo.
TS16: spinal ganglion	Strong		3E/F
TS16: embryo mesenchyme	Present	Regionally restricted	3E/F	Expression could be detected in the somitic mesoderm.
TS16: embryo	Present	Single cells	3E/F	Groups of labelled cells (possibly part of the alimentary system) located between the dorsal aorta and the foregut at the level of the forelimb bud were found one or two somites more anteriorly than the most rostrally expressing somite.
TS16: gut	Present	Regionally restricted	3E/F	Transcripts were found in the mesoderm surrounding the midgut.
TS16: hindgut epithelium	Present		3E/F
TS16: hindgut mesenchyme	Present		3E/F
TS16: hindgut	Present	Regionally restricted	3E/F	The tailgut mesenchyme and epithelium were labelled.
TS16: forelimb bud mesenchyme	Present	Graded	3E/F	Expression was more prominent in the posterior and dorsal part of the forelimb bud. In the anterior part, expression was restricted to dorsally and distally located cells, while more posteriorly, the boundary was located progressively more ventrally.
TS16: forelimb bud ectoderm	Absent		3E/F
TS16: mesonephric tubule	Strong		3E/F
TS16: nephric duct	Strong		3E/F

Structure	Level	Pattern	Image	Note
TS16: central nervous system	Present	Regionally restricted	3G/H	The domain of Hox-2.3 expression started in the upper spinal cord and extended to the caudal end of the embryo.
TS16: spinal ganglion	Strong		3G/H
TS16: embryo mesenchyme	Present	Regionally restricted	3G/H	Expression could be detected in the somitic mesoderm.
TS16: gut	Present	Regionally restricted	3G/H	Transcripts were found in the mesoderm surrounding the midgut.
TS16: hindgut epithelium	Present		3G/H
TS16: hindgut mesenchyme	Present		3G/H
TS16: hindgut	Present	Regionally restricted	3G/H	The tailgut mesenchyme and epithelium were labelled.
TS16: mesonephric tubule	Strong		3G/H
TS16: nephric duct	Strong		3G/H

Structure	Level	Pattern	Image	Note
TS16: forelimb bud ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	4A/B	The domain of Hox-2.3 expression started in the upper spinal cord and extended to the caudal end of the embryo.
TS17: trunk mesenchyme	Present	Regionally restricted	4A/B	Expression was detected in the unsegmented or lateral plate mesoderm within the AP domain of expression defined in the somitic mesoderm.
TS17: embryo	Present	Single cells	4A/B	Groups of labelled cells (possibly part of the alimentary system) located between the dorsal aorta and the foregut at the level of the forelimb bud were found one or two somites more anteriorly than the most rostrally expressing somite.
TS17: mesonephric tubule	Strong		4A/B

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	5A/B	The anterior limit of transcript accumulaton was located in the posterior myelencephalon and thus seemed to be shifted rostrally compared to 2 days earlier.
TS20: spinal cord	Present	Graded	5A/B	Transcript accumulation was enhanced in the dorsal-most area and in a dorsolateral strip of cells, while ventrolaterally no expression was found.
TS20: tail dermomyotome	Absent		5A/B
TS20: axial skeleton	Present	Graded	5A/B	Expression started rostrally in the posterior half of the 7th, or last cervical prevertebra (C7), increased in the next one (T1) and remained detectable up to the most caudal one (not specified).
TS20: tail sclerotome	Weak		5A/B	The level of expression in the sclerotomes decreased compared to earlier somite stages, and they were found to express Hox-2.3 at a much lower level than cells in the neural tube.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	5C/D	Transcript accumulation was enhanced in the dorsal-most area and in a dorsolateral strip of cells, while ventrolaterally no expression was found.
TS20: spinal ganglion	Weak		5C/D	The signal was decreased compared to the signal in the CNS.
TS20: tail dermomyotome	Absent		5C/D
TS20: axial skeleton	Present	Graded	5C/D	Expression started rostrally in the posterior half of the 7th, or last cervical prevertebra (C7), increased in the next one (T1) and remained detectable up to the most caudal one (not specified).
TS20: tail sclerotome	Weak		5C/D	The level of expression in the sclerotomes decreased compared to earlier somite stages, and they were found to express Hox-2.3 at a much lower level than cells in the neural tube.
TS20: nephric duct	Present		5C/D
TS20: primitive collecting duct	Present		5C/D
TS20: ureter	Present		5C/D

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Graded	6E , 6C	Transcript accumulation was enhanced in the dorsal-most area and in a dorsolateral strip of cells, while ventrolaterally no expression was found.
TS20: spinal cord ventricular layer	Present		6E , 6C

Structure	Level	Pattern	Image	Note
TS20: spinal ganglion	Weak		7A/B	The signal was decreased compared to the signal in the CNS
TS20: embryo	Present	Single cells	7A/B	A group of labelled cells, located lateroventrally to the dorsal aorta could possibly represent sympathetic cells on their way to the adrenals where they will form the medulla (chromaffin cells).
TS20: dorsal mesogastrium	Present	Regionally restricted	7A/B	RNA is restricted to the dorsal mesogastrium and to the dorsolateral part of the mesodermal component of the stomach wall.
TS20: stomach	Present	Regionally restricted	7A/B	RNA is restricted to the dorsal mesogastrium and to the dorsolateral part of the mesodermal component of the stomach wall.

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Absent
TS20: limb	Absent
TS20: sympathetic ganglion	Absent
TS20: gut	Present

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Graded	6A	Expression was slightly enhanced in the dorsal region. Results are representative for the thoracic and lumber regions of the neural tube.

Structure	Level	Pattern	Image	Note
TS15: mesonephros	Present

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Graded	6B	More transcripts were located dorsally and ventrally than in between. Results are representative for the thoracic and lumber regions of the neural tube.
TS17: spinal ganglion	Strong		6B

Structure	Level	Pattern	Image	Note
TS17: stomach	Present

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	6D , 6F	Transcripts were more abumdant dorsally and medially in and around the ventricular zone than in the lateralmost region. Results are representative for the thoracic and lumber regions of the spinal cord.
TS22: spinal cord ventricular layer	Present		6F , 6D

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Single cells	8A/B	A group of labelled cells, located lateroventrally to the dorsal aorta could possibly represent sympathetic cells on their way to the adrenals where they will form the medulla (chromaffin cells).
TS22: dorsal mesogastrium	Moderate	Regionally restricted	8A/B	RNA is restricted to the dorsal mesogastrium and to the dorsolateral part of the mesodermal component of the stomach wall.
TS22: stomach	Moderate	Regionally restricted	8A/B	RNA is restricted to the dorsal mesogastrium and to the dorsolateral part of the mesodermal component of the stomach wall.
TS22: collecting duct	Present		8A/B
TS22: nephric duct	Present		8A/B
TS22: paramesonephric duct of male	Present		8A/B
TS22: ureter	Present		8A/B

Structure	Level	Pattern	Image	Note
TS22: axial skeleton	Present	Regionally restricted		Expression in the prevertebrae and intervertebral disks was detected, the transcripts being localized around the chondrification centra and at the junction between the ribs and the prevertebrae, and no signal being detected in the chondrification centra.
TS22: gut	Present
TS22: limb	Absent