Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:55780
Borycki AG, et al., Sonic hedgehog controls epaxial muscle determination through Myf5 activation. Development. 1999 Sep;126(18):4053-63
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:1343822
|
Gene symbol:
|
Myod1
|
Gene name:
|
myogenic differentiation 1
|
Probe:
|
Myod1 probe6
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1I,J:
embryonic day 10.5
(more )
Specimen
1I,J:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermomyotome |
Present |
Graded |
1J
,
1I
|
In interlimb somites expression is higher in the hypaxial region than in the epaxial region.
|
Specimen
1K,L:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk dermomyotome |
Present |
Regionally restricted |
1L
,
1K
|
Expression in the epaxial region is missing. Expression in the hypaxial region is normal.
|
TS17: branchial arch |
Present |
|
1L
,
1K
|
Expression in this mutant is the same as in wild-type embryos.
|
TS17: limb |
Present |
|
1L
,
1K
|
Expression in this mutant is the same as in wild-type embryos.
|
TS17: midbrain |
Absent |
|
1L
,
1K
|
Expression is missing in the head presumably due to midbrain defects.
|
|