Probe:	Probe 2
Probe preparation:	labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Strong	Regionally restricted	2A	Strong expression was seen in the mandibular process of the first branchial arch, especially more posterior parts.
TS17: 2nd branchial arch	Strong	Regionally restricted	2A	Strong expression was seen in the anterior one-third of the second arch.
TS17: limb	Strong		2A

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Strong	Regionally restricted	2C , 2B	Strong expression was seen in the mandibular process of the first branchial arch, especially more posterior parts. Expression was continuous from the left to right sides.
TS17: 2nd branchial arch	Strong	Regionally restricted	2B , 2C	Strong expression was seen in the anterior one-third of the second arch. The posterior boundary of expression was sharp.
TS17: 1st branchial groove	Strong		2B , 2C
TS17: branchial arch	Present	Regionally restricted	2C , 2B	Expression was absent from the epithelium overlying the arch.

Structure	Level	Pattern	Image	Note
TS17: latero-nasal process	Strong		3A
TS17: medial-nasal process	Strong		3A
TS17: trunk	Present	Regionally restricted	3A	Expression was detected in the body wall adjacent to labeling in the limb.
TS17: nasal pit epithelium	Absent		3A
TS17: limb	Strong		3A

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	3B	Expression was detected only within medial parts of the first arch and, apart from the rostral-most limits of this arch, was continuous from the left to right sides.
TS17: 1st branchial arch maxillary component	Absent		3B

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	3C	Expression was detected only within medial parts of the first arch and, apart from the rostral-most limits of this arch, was continuous from the left to right sides.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch	Present	Regionally restricted	3D	Expression was detected both medially and laterally.
TS17: pharynx	Present	Regionally restricted	3D	Expression was detected in the floor of the pharynx.
TS17: trunk	Present	Regionally restricted	3D	Expression was detected in the thoracic body wall adjacent to labeling in the limb overlying the heart but not within more dorsal or ventral regions. Expression in the body wall did not extend continuously between the limbs.
TS17: otocyst epithelium	Present	Regionally restricted	3D	Expression was detected in a discrete ventral region of the otic vesicle epithelium.
TS17: forelimb bud	Strong	Regionally restricted	3D	The bulk of labeling was proximal, ventral and anterior. This domain therefore excludes that part of the limb that includes the zone of polarizing activity.

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial arch	Absent		3E
TS17: 4th branchial arch	Absent		3E
TS17: 2nd branchial arch	Present	Regionally restricted	3E	Expression was detected both medially and laterally, with medially located expression extending posteriorly beyond the limits of more lateral labeling.
TS17: pharynx	Present	Regionally restricted	3E	Expression was detected in the floor of the pharynx.
TS17: trunk	Present	Regionally restricted	3E	Expression was detected in the thoracic body wall adjacent to labeling in the limb overlying the heart but not within more dorsal or ventral regions. Expression in the body wall did not extend continuously between the limbs.
TS17: forelimb bud	Strong	Regionally restricted	3E	The bulk of labeling was proximal, ventral and anterior. This domain therefore excludes that part of the limb that includes the zone of polarizing activity.
TS17: otocyst epithelium	Present	Regionally restricted	3E	Expression was detected in a discrete ventral region of the otic vesicle epithelium.

Structure	Level	Pattern	Image	Note
TS17: 3rd branchial arch	Absent		3F
TS17: 4th branchial arch	Absent		3F
TS17: trunk	Present	Regionally restricted	3F	Expression was detected in the thoracic body wall adjacent to labeling in the limb overlying the heart but not within more dorsal or ventral regions. Expression in the body wall did not extend continuously between the limbs.
TS17: forelimb bud	Strong	Regionally restricted	3F	The bulk of labeling was proximal, ventral and anterior. This domain therefore excludes that part of the limb that includes the zone of polarizing activity.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Strong	Regionally restricted	4B	Expression was generally seen to lie medial to that of Dlx1 (see Assay MGI:1343788). In rostral parts, transcripts occupied apparently exclusive and complementary domains. However, in more posterior parts there was apparent overlap of expression.
TS17: 1st branchial arch maxillary component	Absent		4B

Structure	Level	Pattern	Image	Note
TS20: tongue	Strong	Regionally restricted	5A	Expression was detected in parts of the tongue.
TS20: nasal cavity	Present	Regionally restricted	5A	The earlier expression around the olfactory pit had developed into an extensive zone of labeled mesenchyme surrounding each nasal passage. This zone of expression extended continuously into the ingrowing palatal shelves.
TS20: palatal shelf	Present		5A
TS20: nasal cavity epithelium	Absent		5A

Structure	Level	Pattern	Image	Note
TS20: tongue	Strong	Regionally restricted	5B	Expression was detected in parts of the tongue.
TS20: inner ear	Present	Regionally restricted	5B	Expression was detected in a discrete ventral region of the otic vesicle. Expression appeared to be confined solely to the epithelium.

Structure	Level	Pattern	Image	Note
TS20: embryo	Strong	Regionally restricted	5C	Strong labeling persisted within the hyomandibular cleft.
TS20: mandible primordium	Strong		5C	Strong labeling was detected in the lower jaw.

Structure	Level	Pattern	Image	Note
TS20: hip	Strong	Patchy	5D	Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing hip, tibia and fibula.
TS20: limb	Strong	Patchy	5D	Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing hip, tibia and fibula.
TS20: lower leg	Strong	Patchy	5D	Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing hip, tibia and fibula.
TS20: rib pre-cartilage condensation	Strong	Patchy	5D	Expression was detected around the lateral and ventral, but not dorsal, parts of the developing ribs. Expression around the ribs was excluded from the condensing skeletal elements.
TS20: body wall	Present	Regionally restricted	5D	Expression was maintained within the lateral body wall.

Structure	Level	Pattern	Image	Note
TS20: mandible primordium	Strong		6B	Strong labelling was detected in the lower jaw.
TS20: tongue	Strong	Regionally restricted	6B	The ventral-most parts of the tongue and epithelium overlying the tongue were not labelled. There was a sharp boundary at the anatomical boundary between the body and root of the tongue between labelled anterior and non-labelled posterior parts.
TS20: thymus primordium	Present		6B	A continuous tract of labelled tissues was seen to extend posteriorly from the base of the thyroid duct to below, and around, the aortic arch. These tissues were tentatively identified as the thryoid and thymus glands.
TS20: thyroid gland	Present		6B	A continuous tract of labelled tissues was seen to extend posteriorly from the base of the thyroid duct to below, and around, the aortic arch. These tissues were tentatively identified as the thryoid and thymus glands.
TS20: thyroglossal duct	Present	Regionally restricted	6B	Expression extended posteriorly from the base of the thyroid duct, but was absent from the epithelial tissue lining the anterior edge of the thyroid duct.

Structure	Level	Pattern	Image	Note
TS22: nose	Strong	Regionally restricted	7A	Strong labelling persisted around the nasal passages.
TS22: nasal cavity epithelium	Absent		7A
TS22: lower jaw	Strong		7A
TS22: palatal shelf	Strong		7A
TS22: Meckel's cartilage	Weak		7A	Expression became markedly reduced in central parts of the mandible already transformed into cartilage.
TS22: forelimb	Present	Spotted	7A	Expression within the forelimb was reduced to a few flecks, seen mainly within the shoulder and wrist regions.
TS22: shoulder	Present	Spotted	7A	Expression within the forelimb was reduced to a few flecks, seen mainly within the shoulder and wrist regions.
TS22: diencephalon	Present	Regionally restricted	7A	A small region of tissue within the floor of the diencephalon showed expression.
TS22: cochlear duct epithelium	Present	Regionally restricted	7A	Labelling persisted within discrete regions of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Regionally restricted	7B	Labelling persisted within discrete regions of the cochlear duct. Labelling was restricted to part of the wall adjacent to, but not including, the sensory organ of Corti.

Structure	Level	Pattern	Image	Note
TS22: head mesenchyme	Strong	Regionally restricted	7C	Strong expression was seen in mesenchymal tissues around the developing eustachian tube and base of the auditory meatus.
TS22: malleus cartilage condensation	Strong		7C
TS22: incus cartilage condensation	Absent		7C
TS22: stapes cartilage condensation	Absent		7C
TS22: cochlear duct epithelium	Present	Regionally restricted	7C	Labelling persisted within discrete regions of the cochlear duct.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Absent

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Absent

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Absent
TS20: forearm	Present	Patchy		Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing shoulder, radius and ulna.
TS20: shoulder	Present	Patchy		Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing shoulder, radius and ulna.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Absent			Expression was absent in the central nervous system except the small area in the diencephalon discussed in Fig. 7A.
TS22: midbrain	Absent			Expression was absent in the central nervous system except the small area in the diencephalon discussed in Fig. 7A.
TS22: spinal cord	Absent			Expression was absent in the central nervous system except the small area in the diencephalon discussed in Fig. 7A.
TS22: telencephalon	Absent			Expression was absent in the central nervous system except the small area in the diencephalon discussed in Fig. 7A.

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent		8A , 8B
TS15: forelimb bud	Absent		8B , 8A

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Patchy	9A	Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing ankle and wrist.
TS20: hindlimb	Present	Patchy	9A	Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing ankle and wrist.
TS20: limb digit	Absent		9A

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Patchy	9B	Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing ankle and wrist.
TS20: hindlimb	Present	Patchy	9B	Goosecoid expression was restricted within patches that lay mainly between, rather than within, the mesenchymal condensations destined to give rise to skeletal elements. Expression was seen within the region of the developing ankle and wrist.
TS20: limb digit	Absent		9B