Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:21616
Braun T, et al., MyoD expression marks the onset of skeletal myogenesis in Myf-5 mutant mice. Development. 1994 Nov;120(11):3083-92
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1343706
|
|
Gene symbol:
|
Myod1
|
|
Gene name:
|
myogenic differentiation 1
|
|
Probe:
|
Myod cDNA4
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
1E,F:
embryonic day 9.5
(more  )
Note: 22-somite stage.
Specimen
1E,F:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
22-somite stage.
|
Specimen
1G,H:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
22-somite stage.
|
Specimen
2A,B:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
35-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk somite |
Weak |
Regionally restricted |
2A/B
|
Expression is in some somites.
|
Specimen
4A,B:
embryonic day 11.0
(more )
Note: 40-somite embryo.
Specimen
4A,B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
40-somite embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk somite |
Present |
Regionally restricted |
4A/B
|
Expression in this mutant is the same as in wild-type embryos. Not all somites express at this stage.
|
Specimen
4E,F:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
40-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk somite |
Strong |
Regionally restricted |
4E/F
|
Not all somites express at this stage.
|
Specimen
5A,B:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
37-somite stage.
|
Specimen
5C:
embryonic day 10.5
(more )
Note: 37-somite stage.
Specimen
5C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
37-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
5C/F
|
Expression in the myotome is inferred from the text.
|
Specimen
6C,D:
(close )
|
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
6C/D
|
Expression is in muscle cells. Expression is the same as in wild-type embryos.
|
Specimen
6G,H:
embryonic day 11.5
(more )
Specimen
6G,H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
6G/H
|
Expression is in muscle cells.
|
|
Analysis Tools