Gene Expression Data
Assay Details
Assay
|
Reference:
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J:21616
Braun T, et al., MyoD expression marks the onset of skeletal myogenesis in Myf-5 mutant mice. Development. 1994 Nov;120(11):3083-92
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1343424
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Gene symbol:
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Myog
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Gene name:
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myogenin
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Probe:
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Myog cDNA13
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1A,B:
embryonic day 9.5
(more )
Note: 22-somite stage.
Specimen
1A,B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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22-somite stage.
|
Specimen
1C,D:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
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Mutant Allele(s):
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Myf5tm1Jae/Myf5tm1Jae
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
22-somite stage.
|
Specimen
2C,D:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
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Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Note:
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35-somite stage.
|
Specimen
4C,D:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
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Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Note:
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40-somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk somite |
Present |
Regionally restricted |
4C/D
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Expression is only in somites that express Myod1.
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Specimen
4G,H:
embryonic day 11.0
(more )
Note: 40-somite stage.
Specimen
4G,H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
40-somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: trunk somite |
Present |
Regionally restricted |
4G/H
|
Expression occurs before Myod1 expression in most caudal somites.
|
Specimen
6A,B:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: limb |
Present |
Regionally restricted |
6A/B
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Expression is in muscle cells. Expression in this mutant is the same as in wild-type embryos.
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Specimen
6E,F:
embryonic day 11.5
(more )
Specimen
6E,F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: limb |
Present |
Regionally restricted |
6E/F
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Expression is in muscle cells.
|
Specimen
7B:
(close )
Genetic Background:
|
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
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Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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32-somite stage.
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Specimen
7E:
embryonic day 10.0
(more )
Note: 32-somite stage.
Specimen
7E:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
32-somite stage.
|
|