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Gene Expression Data
Assay Details
Assay
Reference: J:53337 Yuan SS, et al., Cloning and functional studies of a novel gene aberrantly expressed in RB-deficient embryos. Dev Biol. 1999 Mar 1;207(1):62-75
Assay type: Western blot
MGI Accession ID: MGI:1343382
Gene symbol: Robo3
Gene name: roundabout guidance receptor 3
Antibody: rabbit anti-ECD
Assay notes: Fractionation experiments elsewhere in the paper indicated that the protein is in the membrane.
Results
Image: 5b left ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 210 kDa 160 kDa (a) Genetic BackgroundMutant Allele(s)Sex Note
1 E11.5 TS19: future spinal cord
TS19: hindbrain
Present Present involves: 129S7/SvEvBrd * C57BL/6 Rb1tm1Brd/Rb1tm1BrdNot Specified
2 Control
3 E11.5 TS19: future spinal cord
TS19: hindbrain
Present Present involves: 129S7/SvEvBrd * C57BL/6 Rb1tm1Brd/Rb1tm1BrdNot Specified Immunoprecipitation was performed using rabbit anti-ECD prior to Western blot analysis.
4 Control
Notes:
(a) The ~160-kDa protein band whose identity is unknown was always present in immunoblots using rabbit anti-ECD or mouse anti-ICD antisera in various mouse tissues.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory