Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45709
Melloy PG, et al., No turning, a mouse mutation causing left-right and axial patterning defects. Dev Biol. 1998 Jan 1;193(1):77-89
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1343322
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Gene symbol:
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Lefty1
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Gene name:
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left right determination factor 1
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Probe:
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lefty probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5C:
embryonic day 8.0
(more  )
Note: 3-5 somites.
Specimen
5C:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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Specimen
5D:
embryonic day 8.0; nt/ nt
(more )
Note: 3-5 somites.
Specimen
5D:
(close )
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Genetic Background:
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CD-1
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Mutant Allele(s):
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nt/nt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube floor plate |
Absent |
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5D
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
5D
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Lefty is bilaterally expressed in 4 of 7 nt embryos examined. In two of these, lefty expression was asymmetric, one showing higher expression in the left and ther other in the right lateral plate mesoderm. Lefty was absent in the other embryos.
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Specimen
5E:
embryonic day 8.0; nt/ nt
(more )
Note: 3-5 somites.
Specimen
5E:
(close )
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Genetic Background:
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CD-1
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Mutant Allele(s):
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nt/nt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-5 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube floor plate |
Absent |
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5E
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
5E
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Lefty is bilaterally expressed in 4 of 7 nt embryos examined. In two of these, lefty expression was asymmetric, one showing higher expression in the left and ther other in the right lateral plate mesoderm. Lefty was absent in the other embryos.
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Analysis Tools