Probe:	Tgfb2 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube cardiac muscle	Present		1B

Structure	Level	Pattern	Image	Note
TS12: pericardio-peritoneal component mesothelium	Strong		1E/F	Expression is in the caudal splanchnic epithelium lining the left and right pericardio-peritoneal coelema.

Structure	Level	Pattern	Image	Note
TS12: somatopleure	Present	Regionally restricted	1G/H	Expression is in the epithelia.
TS12: splanchnopleure	Present	Regionally restricted	1G/H	Expression is in the epithelia.
TS12: foregut diverticulum endoderm	Present	Regionally restricted	1G/H	Expression is ventral.
TS12: vitelline vein	Weak	Regionally restricted	1G/H	Expression is in the promyocardium.

Structure	Level	Pattern	Image	Note
TS12: sinus venosus	Weak	Regionally restricted	1I/J	Expression is in the promyocardium.
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	1I/J	Expression is in the ventral foregut endoderm and in the thyroid diverticulum.
TS12: splanchnopleure	Strong	Regionally restricted	1I/J	Expression is in the splanchnic epithelium.

Structure	Level	Pattern	Image	Note
TS12: pericardial cavity	Strong	Regionally restricted	1K/L	Expression is in tissues all around the pericardial cavity.
TS12: somatopleure	Strong	Regionally restricted	1K/L	Expression is in the splanchnic and somatic epithelia.
TS12: splanchnopleure	Strong	Regionally restricted	1K/L	Expression is in the splanchnic and somatic epithelia.
TS12: foregut diverticulum endoderm	Strong	Regionally restricted	1K/L	Expression is in the ventral foregut endoderm and in the thyroid diverticulum.
TS12: primitive heart tube	Present	Graded	1K/L	There is a rostrocaudal gradient of expression so that in the outflow tract and primitive ventricle expression is barely detectable.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	1M/N	Expression is in the myocardial cells.
TS12: dorsal mesocardium	Present		1M/N
TS12: dorsal aorta	Present	Regionally restricted	1M/N	Expression is in the myocardial cells in the distal region of the aortic sac.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		3B , 3A
TS28: uterus	Present	Regionally restricted	3B , 3A	Expression is in the uterine epithelium.

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber cardiac muscle	Present		4A/B	Expression is in the myocardium of the atrioventricular region.
TS13: primitive ventricle cardiac muscle	Present		4A/B	Expression is in the myocardium of the atrioventricular region.
TS13: outflow tract	Present	Regionally restricted	4A/B	Expression is in some myocardial cells.

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber cardiac muscle	Present		4D/E	Expression is in the myocardium of the atrioventricular region.
TS13: primitive ventricle cardiac muscle	Present		4D/E	Expression is in the myocardium of the atrioventricular region.
TS13: septum transversum	Present		4D/E

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber cardiac muscle	Present		4G/H	Expression is in the myocardium of the atrioventricular region.
TS13: primitive ventricle cardiac muscle	Present		4G/H	Expression is in the myocardium of the atrioventricular region.
TS13: septum transversum	Present		4G/H

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber cardiac muscle	Present		4J/K	Expression is in the myocardium of the atrioventricular region.
TS13: primitive ventricle cardiac muscle	Present		4J/K	Expression is in the myocardium of the atrioventricular region.
TS13: septum transversum	Present		4J/K
TS13: intraembryonic coelom	Present	Regionally restricted	4J/K	Expression is in coelomic epithelia.

Structure	Level	Pattern	Image	Note
TS13: common atrial chamber cardiac muscle	Present		4M/N	Expression is in the myocardium of the atrioventricular region.
TS13: primitive ventricle cardiac muscle	Present		4M/N	Expression is in the myocardium of the atrioventricular region.
TS13: lateral plate mesoderm	Present		4M/N

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal	Present	Regionally restricted	6A/B , 6C	Expression is myocardial and is less intense than in 9.5dpc embryos.
TS17: outflow tract	Present	Regionally restricted	6A/B , 6C	Expression is myocardial and is less intense than in 9.5dpc embryos.

Structure	Level	Pattern	Image	Note
TS19: endocardial cushion	Weak	Regionally restricted	6F , 6D/E	Expression is in a band of specialized cardiomyocytes adjacent to the cushion tissue.
TS19: septum transversum	Weak		6D/E , 6F

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Weak	Regionally restricted	6G/H	Expression is in mesenchymal cells.
TS20: cardiac muscle tissue	Absent		6G/H

Structure	Level	Pattern	Image	Note
TS17: trunk dermomyotome	Present	Uniform	7A/B

Structure	Level	Pattern	Image	Note
TS20: tail dermomyotome	Present	Regionally restricted	7E/F	There is expression in the dermatome and a low level of expression in the myotome.

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: future hindbrain floor plate	Present	Regionally restricted		Expression is in a small region of the neuroepithelium that is apposed to the rostral extremity of the foregut.
TS12: future prosencephalon floor plate	Present	Regionally restricted		Expression is in a small region of the neuroepithelium that is apposed to the rostral extremity of the foregut.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial membrane endoderm	Strong	Regionally restricted		Expression is in the caudal portion.
TS15: embryo mesenchyme	Present	Regionally restricted		Expression is in the lateral plate mesoderm of the body walls which extend caudally from the pharyngeal region.
TS15: septum transversum hepatic component	Absent

Structure	Level	Pattern	Image	Note
TS17: 1st branchial pouch endoderm	Strong	Regionally restricted		Expression is in the caudal portion.
TS17: embryo mesenchyme	Present	Regionally restricted		Expression is in the lateral plate mesoderm of the body walls which extend caudally from the pharyngeal region.
TS17: septum transversum	Absent