Probe:	lambda-G14A
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: extraembryonic visceral endoderm	Present		1A
TS10: embryo	Present	Regionally restricted	1A	Expression is present in discrete regions of intraembryonic tissue lateral and subjacent to the neural epithelium.

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted		Expression is present in the mesoderm at the anterior end of the embryo.

Structure	Level	Pattern	Image	Note
TS11: embryo mesenchyme	Present	Regionally restricted	1C	Expression is evident in a split crescent of splanchnopleura.

Structure	Level	Pattern	Image	Note
TS12: intraembryonic coelom	Strong	Regionally restricted	1D	Expression is present immediately caudal to the heart tube, in splanchnic epithelium lining the left and right intraembryonic coelomic cavities.
TS12: embryo mesenchyme	Absent		1D	There is no expression in the somatic mesoderm nor in the more rostral splanchnic mesoderm.
TS12: splanchnopleure	Absent		1D	There is no expression in the somatic mesoderm nor in the more rostral splanchnic mesoderm.
TS12: primitive heart tube	Present	Regionally restricted	1D	Expression is present only in the extreme caudal end of the heart tube.

Structure	Level	Pattern	Image	Note
TS12: intraembryonic coelom	Strong	Regionally restricted	1F	Expression is present immediately caudal to the heart tube, in splanchnic epithelium lining the left and right intraembryonic coelomic cavities.
TS12: embryo mesenchyme	Absent		1F	There is no expression in the somatic mesoderm nor in the more rostral splanchnic mesoderm.
TS12: splanchnopleure	Absent		1F	There is no expression in the somatic mesoderm nor in the more rostral splanchnic mesoderm.
TS12: primitive heart tube	Present	Regionally restricted	1F	Expression is present only in the extreme caudal end of the heart tube.

Structure	Level	Pattern	Image	Note
TS13: septum transversum	Strong		5B , 5A
TS13: sinus venosus	Strong		5B , 5A
TS13: intraembryonic coelom	Strong	Regionally restricted	5B , 5A	Expression is present in the epithelial cells lining the intraembryonic coelomic cavities.
TS13: vitelline vein	Strong	Regionally restricted	5A , 5B	Expression is present in the proximal vitelline veins.
TS13: venous system	Strong	Regionally restricted	5A , 5B	Expression is present in the omphalomesenteric veins.
TS13: head mesenchyme	Weak		5A , 5B
TS13: branchial arch mesenchyme	Weak	Regionally restricted	5A , 5B	Small amounts of staining was evident in pharyngeal arch mesoderm.
TS13: foregut diverticulum endoderm	Absent		5A , 5B
TS13: paraxial mesenchyme	Absent		5B , 5A	There is no expression evident in somatic mesoderm.

Structure	Level	Pattern	Image	Note
TS16: embryo mesenchyme	Present	Regionally restricted	6A	Expression in present in mesodermal structures immediately caudal to the heart.
TS16: heart	Present		6A

Structure	Level	Pattern	Image	Note
TS19: heart atrium	Present		8A
TS19: heart ventricle	Present		8A
TS19: outflow tract	Present	Regionally restricted	8A	Expression is present in the proximal outflow tract.
TS19: lung	Absent		8A