Probe:	lambda-G14A
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present	Regionally restricted	2B	Expression is present at the anterior end of the embryo.
TS10: extraembryonic visceral endoderm	Present		2B
TS10: trophectoderm	Absent		2B

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent		2B

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	2E	Expression is present the extreme caudal end of the heart tube.
TS12: extraembryonic visceral endoderm	Present		2E
TS12: neural ectoderm	Absent		2E

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent		2E

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present	Regionally restricted	3B , 3A	Expression is present in the extreme caudal end of the heart tube.
TS12: yolk sac endoderm	Present		3A , 3B
TS12: intraembryonic coelom	Present	Regionally restricted	3B , 3A	Expression is present in the epithelial cells lining the ventrocaudal portion of the coelomic cavity.

Structure	Level	Pattern	Image	Note
TS13: septum transversum	Strong		5D
TS13: sinus venosus	Strong		5D
TS13: intraembryonic coelom	Strong	Regionally restricted	5D	Expression is present in epithelial cells lining the intraembryonic coelomic cavities.
TS13: vitelline vein	Strong	Regionally restricted	5D	Expression is present in proximal vitelline veins.
TS13: venous system	Strong	Regionally restricted	5D	Expression is present in omphalomesenteric veins.
TS13: endocardial tube	Moderate		5D	Expression is present in the endocardium.
TS13: outflow tract	Moderate	Regionally restricted	5D	Expression is present in the outflow tract; it appears to stop abruptly in the distal outflow tract.
TS13: bulbus cordis rostral half cardiac muscle	Moderate		5D	Expression levels in the caudal myocardium are higher than in the rostral myocardium.
TS13: bulbus cordis caudal half cardiac muscle	Strong		5D	Expression levels in the caudal myocardium are higher than in the rostral myocardium.
TS13: dorsal aorta	Absent		5D	No expression was detected in the aortic sac or dorsal aortae.
TS13: foregut diverticulum endoderm	Absent		5D
TS13: paraxial mesenchyme	Absent		5D	No expression was evident in somatic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Moderate		5E	Expression is present in the endocardium.
TS13: outflow tract	Moderate	Regionally restricted	5E	Expression is present in the proximal outflow tract; it appears to stop abruptly in the distal outflow tract.
TS13: bulbus cordis rostral half cardiac muscle	Moderate		5E	Expression levels in the caudal myocardium are higher than in the rostral myocardium.
TS13: bulbus cordis caudal half cardiac muscle	Strong		5E	Expression levels in the caudal myocardium are higher than in the rostral myocardium.
TS13: dorsal aorta	Absent		5E	No expression of was detected in the aortic sac or dorsal aortae.

Structure	Level	Pattern	Image	Note
TS16: cardiac muscle tissue	Present		7B	Expression is present in the endocardial and myocardial layers of the heart.
TS16: endocardial lining	Present		7B	Expression is present in the endocardial and myocardial layers of the heart.
TS16: 1st branchial arch mesenchyme	Present		7B	Expression is present in pharyngeal arch mesoderm.

Structure	Level	Pattern	Image	Note
TS16: endocardial cushion	Present	Regionally restricted	7F	Expression is present in the endocardial cushion tissue of the AV and outflow tract regions.