Probe:	Hox2.1 Probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Identical results were obtained using the probe MGI:1341314.

Structure	Level	Pattern	Image	Note
TS20: lung	Weak		4B
TS20: spinal cord	Weak	Uniform	4B
TS20: brain	Absent		4B	Expression was not detected anterior to the hindbrain boundary.
TS20: central nervous system	Present	Regionally restricted	4B	Expression is caudal to a boundary in the posterior myelencephalon.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Strong	Regionally restricted	5B	Expression is in both central and ventral regions.

Structure	Level	Pattern	Image	Note
TS20: C1 vertebral cartilage condensation	Absent		6B
TS20: C2 vertebral cartilage condensation	Absent		6B
TS20: C3 vertebral cartilage condensation	Absent		6B
TS20: C4 vertebral cartilage condensation	Absent		6B
TS20: C5 vertebral cartilage condensation	Weak		6B	Low levels of transcripts in ventral parts of prevertebra 5 in some sections.
TS20: C6 vertebral cartilage condensation	Present		6B
TS20: C7 vertebral cartilage condensation	Present		6B
TS20: T1 vertebral cartilage condensation	Strong		6B
TS20: T2 vertebral cartilage condensation	Strong		6B
TS20: T3 vertebral cartilage condensation	Strong		6B
TS20: T4 vertebral cartilage condensation	Strong		6B
TS20: T5 vertebral cartilage condensation	Strong		6B
TS20: T6 vertebral cartilage condensation	Strong		6B
TS20: T7 vertebral cartilage condensation	Strong		6B
TS20: T10 vertebral cartilage condensation	Present		6B
TS20: T8 vertebral cartilage condensation	Present		6B
TS20: T9 vertebral cartilage condensation	Present		6B
TS20: L1 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: L2 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: L3 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: L4 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: L5 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: L6 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: S1 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: S2 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: S3 vertebral pre-cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: S4 vertebral pre-cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: T11 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: T12 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.
TS20: T13 vertebral cartilage condensation	Weak		6B	Low levels of labeling in all prevertebrae posterior to prevertebra 17.

Structure	Level	Pattern	Image	Note
TS20: lung	Weak	Regionally restricted	8B	Expression is confined to the outer mesodermal component.
TS20: trachea	Trace		8B
TS20: esophagus epithelium	Present		8B

Structure	Level	Pattern	Image	Note
TS20: stomach epithelium	Present	Regionally restricted	9B	Expression is in the anterior part of the stomach.
TS20: stomach mesenchyme	Present		9B
TS20: stomach	Present	Regionally restricted	9B	Expression is in the dorsal but not the ventral parts of the stomach wall.
TS20: mesonephros	Strong		9B
TS20: metanephros	Strong		9B
TS20: testis	Present	Regionally restricted	9B	There is no expression in the testis cord.

Structure	Level	Pattern	Image	Note
TS20: gut	Present	Regionally restricted	10B	Expression is in the mesodermal components of the posterior part of the gut in some but not all loops.