Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54746
Matise MP, et al., Ventral midline cells are required for the local control of commissural axon guidance in the mouse spinal cord. Development. 1999 Aug;126(16):3649-59
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1340744
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Gene symbol:
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Nrcam
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Gene name:
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neuronal cell adhesion molecule
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Antibody:
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anti-Nr-CAM
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
5.A:
embryonic day 11.0
(more  )
Specimen
5.A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
5A
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Staining is detected on commissural axons as they project toward the ventral midline. As commissural axons enter the ventral commissure, expression becomes highly enriched. Weak expression is seen in the ventral funiculus on postcommissural axons.
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| TS18: neural tube floor plate |
Present |
Regionally restricted |
5A
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Specimen
5.B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
5B
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Precommissural level of expression was similar to that in wild-type; however, expression did not increase at the ventral midline. Weak expression was seen in some regions of the ventral funiculus.
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Analysis Tools