Gene Expression Data
Assay Details
Assay
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Reference:
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J:56093
Bruneau BG, et al., Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome. Dev Biol. 1999 Jul 1;211(1):100-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1340489
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Gene symbol:
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Tbx5
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Gene name:
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T-box 5
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Probe:
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Tbx5 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.0
(more )
Note: Two-somite embryo. Frontal view.
Specimen
2A:
(close )
Genetic Background:
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(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Two-somite embryo. Frontal view.
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Structure |
Level |
Pattern |
Image |
Note |
TS12: heart |
Present |
Regionally restricted |
2A
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Expression is in the cardiac cresent.
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Specimen
2B:
embryonic day 8.25
(more )
Note: Ventral view.
Specimen
2B:
(close )
Genetic Background:
|
(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 8.25
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Ventral view.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: common atrial chamber |
Strong |
|
2B
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Expression was robust in the very posterior portion of the heart, which is destined to become the sinus venosa and the atria.
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TS12: sinus venosus |
Strong |
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2B
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Expression was robust in the very posterior portion of the heart, which is destined to become the sinus venosa and the atria.
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TS12: primitive heart tube cardiac muscle |
Present |
Regionally restricted |
2B
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Expression is in the myocardium that encircles the endocardial tube.
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Specimen
2C:
embryonic day 8.5
(more )
Note: Ventral view.
Specimen
2C:
(close )
Genetic Background:
|
(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 8.5
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Ventral view.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: common atrial chamber |
Present |
|
2C
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Expression was localized exclusively in the posterior structures of the heart that give rise to the atria and the sinus venosa.
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TS13: sinus venosus |
Present |
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2C
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Expression was localized exclusively in the posterior structures of the heart that give rise to the atria and the sinus venosa.
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Specimen
2D:
embryonic day 8.5
(more )
Note: Right side lateral view.
Specimen
2D:
(close )
Genetic Background:
|
(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 8.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Right side lateral view.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: common atrial chamber |
Present |
|
2D
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Expression was localized exclusively in the posterior structures of the heart that give rise to the atria and the sinus venosa.
|
TS12: sinus venosus |
Present |
|
2D
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Expression was localized exclusively in the posterior structures of the heart that give rise to the atria and the sinus venosa.
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Specimen
2E:
embryonic day 9.0
(more )
Note: Viewed from the right side.
Specimen
2E:
(close )
Genetic Background:
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(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 9.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Viewed from the right side.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: primitive ventricle |
Present |
Regionally restricted |
2E
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Originally expression is in the entire ventricle, but when the ventricles adopt a left-right arrangement, expression is restricted to the left side of the ventricles.
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Specimen
2F:
embryonic day 9.0
(more )
Note: Viewed from the left side.
Specimen
2F:
(close )
Genetic Background:
|
(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 9.0
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
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Viewed from the left side.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: primitive ventricle |
Present |
Regionally restricted |
2F
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Expression is restricted to the left side of the ventricles.
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Specimen
2G:
embryonic day 9.0
(more )
Note: Viewed ventrally.
Specimen
2G:
(close )
Genetic Background:
|
(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 9.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Viewed ventrally.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: primitive ventricle |
Present |
Regionally restricted |
2G
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Expression is restricted to the left side of the ventricles.
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Specimen
2H:
embryonic day 9.5
(more )
Note: Viewed from the left side.
Specimen
2H:
(close )
Genetic Background:
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(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 9.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Viewed from the left side.
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Specimen
2I:
embryonic day 11.5
(more )
Note: Viewed from the front.
Specimen
2I:
(close )
Genetic Background:
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(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Viewed from the front.
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Specimen
2J:
embryonic day 11.5
(more )
Note: Viewed from the back.
Specimen
2J:
(close )
Genetic Background:
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(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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Viewed from the back.
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Specimen
2K:
embryonic day 11.5
(more )
Specimen
2K:
(close )
Genetic Background:
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(Swiss Webster x C57BL/6)F1
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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