Antibody:	anti Pax2 Z-RX2 (71-6000)
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present			In rostral sections of E12 embryos, where the floor plate was present, expression in the heterozygote was the same as in wild-type embryos. Aberrant expression was evident in caudal sections of heterozygous embryos where the floor plate was absent.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted		In rostral sections of E12 embryos, where the floor plate was present, expression in the mutant was the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	1A	Expression was detected in the dorsal three-fourths of the intermediate zone but was undetectable in the more ventral half of the basal plate of the lumbar spinal cord.

Structure	Level	Pattern	Image	Note
TS20: alar columns	Present	Regionally restricted	1C	Expression was detected in the ventral half of the alar plate in the caudal spinal cord. The expression pattern was reiterated along the entire anterior-posterior axis.
TS20: basal columns	Present	Regionally restricted	1C	Expression was detected in the dorsal half but was absent in the ventral half of the basal plate in the caudal spinal cord. The expression pattern was reiterated along the entire anterior-posterior axis.
TS20: spinal cord sulcus limitans	Absent		1C	There was a clear separation at the presumptive sulcus limitans in the caudal spinal cord. The expression pattern was reiterated along the entire anterior-posterior axis.
TS20: spinal cord floor plate	Absent		1C	The expression pattern was described in the caudal spinal cord but was reiterated along the entire anterior-posterior axis.
TS20: spinal cord roof plate	Absent		1C	The expression pattern was described in the caudal spinal cord but was reiterated along the entire anterior-posterior axis.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		1E	Expression pattern varied depending on distance from the floor plate. Expression domains extended progressively more ventrally until a continuous band of expressing cells extended around the ventral midline.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present			In rostral sections, at levels where the floor plate was present, expression in the heterozygote was the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present			In rostral sections, at levels where the floor plate was present, expression in the mutant was the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2A	Rostral to the region lacking a floor plate, expression was detected in the enlarged intermediate zone. It was absent in cells that give rise to the intermediate central gray region and from presumptive motor neuroblasts in the ventrolateral basal plate.
TS21: alar columns	Present	Regionally restricted	2A	Rostral to the region lacking a floor plate, expression was detected in the most dorsal alar plate, which will later form the substantia gelatinosa.
TS21: spinal cord floor plate	Absent		2A
TS21: spinal cord roof plate	Absent		2A

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		2C	Within the caudal region of wild-type E13 embryos, expression was in a pattern reminiscent of more anterior spinal cord at earlier times.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong	Regionally restricted	2E	In mutants, in the absence of the floor plate, expression was very intense at the ventral midline.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2G	In mutants, in the absence of the floor plate, expression was very intense at the ventral midline. In some mutant embryos, expression was present at both the dorsal and ventral midline.

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present		3A	Expression in the mutant was the same as in wild-type embryos.
TS19: nephric duct	Present		3A	Expression in the mutant was the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present
TS19: nephric duct	Present

Structure	Level	Pattern	Image	Note
TS21: mesenchyme of metanephros	Absent		3C	Expression was not detected in the mesenchyme cells surrounding the posterior mesonephric duct.

Structure	Level	Pattern	Image	Note
TS20: mesenchyme of metanephros	Present	Regionally restricted	3D	Expression was detected in the metanephros in the branched ureter and in the condensed mesenchyme surrounding the ureter.
TS20: ureter	Present	Regionally restricted	3D	Expression was detected in the metanephros in the branched ureter and in the condensed mesenchyme surrounding the ureter.

Structure	Level	Pattern	Image	Note
TS21: ureter	Present		3E	Expression was detected in the ureteric bud but was absent in the surrounding cells.

Structure	Level	Pattern	Image	Note
TS21: ureter	Present		3F
TS21: mesenchyme of metanephros	Present	Regionally restricted	3F	Expression was detected in the induced mesenchyme cells of the kidney.