Probe:	Cer-1 probe3
Probe preparation:	labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	4A left	Expression was detected in a stripe, several cell diameters wide on the side of the egg cylinder. The stripe extended proximally from the embryonic/extraembryonic junction almost to the distal tip and was on the opposite side of the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	4A center	Expression continued in a midline stripe on the anterior side.
TS10: mesendoderm	Present	Regionally restricted	4A center	Expression was detected in mesendodermal wings in the anterior region of the streak.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	4A right	Expression continued in a midline stripe on the anterior side.
TS10: mesendoderm	Present	Regionally restricted	4A right	Expression was detected in migrating mesendodermal wings arising in the anterior region of the streak. The domain next to the primitive streak expanded and extended more into the anterior half of the embryo.

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Regionally restricted	4B	Expression was detected in a stripe anteriorly.

Structure	Level	Pattern	Image	Note
TS9: extraembryonic visceral endoderm	Present

Structure	Level	Pattern	Image	Note
TS10: mesendoderm	Present	Regionally restricted	4C	Expression was detected in anterior mesendoderm.

Structure	Level	Pattern	Image	Note
TS10: node	Absent		4D

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	4E	Expression was present in the anterior half of the embryo, was absent from the cardiac progenitor region and no longer extended to the embryonic/extraembryonic junction: it was set back medially and fell away laterally with a relatively sharp boundary.

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present		4H
TS10: embryo mesoderm	Present		4H
TS10: embryo ectoderm	Absent		4H
TS10: node	Absent		4H

Structure	Level	Pattern	Image	Note
TS10: embryo endoderm	Present		4I
TS10: embryo mesoderm	Present		4I
TS10: embryo ectoderm	Absent		4I

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Present	Regionally restricted	4J	Expression was seen in a wedge of mesoderm and endoderm at the anterior tip of the embryo.
TS11: embryo mesoderm	Present	Regionally restricted	4J	Expression was seen in a wedge of mesoderm and endoderm at the anterior tip of the embryo.

Structure	Level	Pattern	Image	Note
TS11: embryo endoderm	Present	Regionally restricted	4K	Expression was seen in a wedge of mesoderm and endoderm at the anterior tip of the embryo. The positive zone clearly excluded cardiogenic mesoderm and its underlying endoderm.
TS11: embryo mesoderm	Present	Regionally restricted	4K	Expression was seen in a wedge of mesoderm and endoderm at the anterior tip of the embryo. The positive zone clearly excluded cardiogenic mesoderm and its underlying endoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	5A	Expression is present in the prechordal and head mesoderm.

Structure	Level	Pattern	Image	Note
TS11: notochordal plate	Present		5B
TS11: embryo mesoderm	Present	Regionally restricted	5B	Expression was detected in head mesoderm.

Structure	Level	Pattern	Image	Note
TS11: node	Absent		5C
TS11: embryo mesoderm	Present	Regionally restricted	5C	Expression was detected in head mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	6A	Expression was reduced to a trace at the anterior ventral midline.
TS11: embryo mesoderm	Present	Regionally restricted	6A	Two stripes of expression were apparent in anterior paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	6B	Two stripes of expression were apparent in anterior paraxial mesoderm.
TS11: mesendoderm	Absent		6B	There is no expression in the anterior mesendoderm.

Structure	Level	Pattern	Image	Note
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	6C	Expression was present in four stripes in paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS13: head paraxial mesenchyme	Present	Regionally restricted	6D	Expression was detected in most or all cells within a paraxial stripe.

Structure	Level	Pattern	Image	Note
TS15: tail paraxial mesenchyme	Present	Regionally restricted	6E	Expression was present in four stripes in the paraxial mesoderm. The weaker anteriormost stripes mark the rostral region of the most recently formed somites, while the stronger posterior stripes are within the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS20: tail somite	Present	Regionally restricted	6F	Expression was present only within nascent and newly formed somites within the tail.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	7_left	In severely affected mutant embryos, expression was restricted to the distal tip and was absent at the embryonic/extraembryonic junction, marked by a prominent constriction.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	7_center	In severely affected mutant embryos, expression was restricted to the distal tip and was absent at the embryonic/extraembryonic junction, marked by a prominent constriction.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	7_right	In the less affected mutant, expression tended more towards the normal pattern, although anterior patterning is still disturbed in these embryos.