Probe:	c52
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo	Trace	Regionally restricted	5A	Expression is detected in wedge shaped domains on either side of the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: node	Weak		5D , 5C
TS11: notochordal plate	Weak		5D , 5C

Structure	Level	Pattern	Image	Note
TS12: head paraxial mesenchyme	Present	Regionally restricted	5E	Expression is detected from the anterior paraxial mesoderm to the middle of the primitive streak.
TS12: primitive streak	Present	Regionally restricted	5E	Expression is detected from the anterior paraxial mesoderm to the middle of the primitive streak.
TS12: trunk paraxial mesenchyme	Present	Regionally restricted	5E	Expression is detected from the anterior paraxial mesoderm to the middle of the primitive streak.

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	5F	Expression is detected on either side of the midline from head to tail.
TS13: neural tube	Present	Regionally restricted	5F	Expression is detected on either side of the midline from head to tail.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch	Present	Regionally restricted	5G	Expression is detected in neural-crest-derived cells of the first branchial arch and in somitomere-derived cells.
TS14: head somite	Present	Regionally restricted	5G	Expression is detected in neural-crest-derived cells of the first branchial arch and in somitomere-derived cells.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Graded	5H	Expression decreses from anterior to posterior.

Structure	Level	Pattern	Image	Note
TS11: node	Strong
TS11: future spinal cord	Strong	Regionally restricted		Expression is detected on either side of the midline.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Graded	6A	Expression is restricted to non-notochordal mesoderm and is higher in paraxial mesoderm, lower in lateral mesoderm.
TS11: embryo ectoderm	Absent		6A	Expression is absent from the epiblast.
TS11: embryo endoderm	Absent		6A
TS11: node	Absent		6A
TS11: primitive streak	Absent		6A
TS11: notochordal process	Absent		6A	Expression is absent from the notochord.

Structure	Level	Pattern	Image	Note
TS13: head somite	Present		6B
TS13: embryo mesenchyme	Present	Regionally restricted	6B	Expression in the lateral mesoderm fades laterally. Expression is also detected in the medial mesoderm.
TS13: lateral plate mesoderm	Present	Regionally restricted	6B	Expression in the lateral mesoderm fades laterally. Expression is also detected in the medial mesoderm.
TS13: paraxial mesenchyme	Present		6B	Expression is present in the somitic mesoderm.

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Present	Regionally restricted	6C	Expression is uniform in all non-notochrdal mesoderm except the heart.
TS14: neural tube	Absent		6C
TS14: notochord	Absent		6C