Probe:	pmet SC1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	For the exact breeding scheme of mutant mice, see the original reference.

Structure	Level	Pattern	Image	Note
TS10: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	1A	Expression is in the ventral portion of the somites at the level of the forelimb buds and in the dorsal tip of a few rostral somites.
TS15: tail	Present		1A

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	1B	There is no expression in the ventral tip at the level of the forelimb. Expression is in the ventral region of thoracic, hindlimb, and tail somites. There is expression in the dorsal tip of the somites.
TS15: forelimb bud	Present	Regionally restricted	1B

Structure	Level	Pattern	Image	Note
TS16: trunk somite	Present	Regionally restricted	1C	There is no expression in the ventral tips at the level of the hindlimb.
TS16: hindlimb bud	Present		1C

Structure	Level	Pattern	Image	Note
TS16: tail somite	Present	Regionally restricted	1E	Expression is in the dorsal and ventral tips of the somites.
TS16: trunk somite	Present	Regionally restricted	1E	Expression is in the dorsal and ventral tips of the somites.
TS16: hindlimb bud	Present		1E

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present		1I

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present	Regionally restricted	1J	Expression is on the ventral side.

Structure	Level	Pattern	Image	Note
TS19: handplate	Present	Regionally restricted	1K	Expression is on the dorsal side in the digit-forming area.

Structure	Level	Pattern	Image	Note
TS19: handplate	Present	Regionally restricted	1L	Expression is on the ventral side in the digit-forming area.

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	2C	Expression is in the ventrolateral tip.

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	2D	Expressing cells localized to the lateral tip of the dermomyotome appeared to detach from the somite domain and accumulate in clusters at the body-limb junction, and then further invade the limb.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud	Present		2E	Expressing cells localized to the lateral tip of the dermomyotome appeared to detach from the somite domain and accumulate in clusters at the body-limb junction, and then further invade the limb.
TS16: trunk dermomyotome	Present		2E	Expression is in the dorsal-medial tip.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	3B	Expression is in a subapical domain on the dorsal and ventral limb side.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present		4A
TS18: hindlimb bud mesenchyme	Present		4A
TS18: trunk somite	Present	Regionally restricted	4A	Expression is in the ventral tip of thoracic and caudal somites and in the dorsal tip of nearly all somites.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present		4B
TS18: hindlimb bud mesenchyme	Present		4B
TS18: trunk somite	Present	Regionally restricted	4B	Expression is in the ventral tip of thoracic and caudal somites.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present		4C
TS18: hindlimb bud mesenchyme	Present		4C

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present		4D	Expression in this mutant is the same as in wild-type embryos.
TS18: hindlimb bud mesenchyme	Present		4D	Expression in this mutant is the same as in wild-type embryos.
TS18: trunk somite	Present	Regionally restricted	4D	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present		4E	Expression in this mutant is the same as in wild-type embryos.
TS18: hindlimb bud mesenchyme	Present		4E	Expression in this mutant is the same as in wild-type embryos.
TS18: trunk somite	Present	Regionally restricted	4E	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present		4F	Expression in this mutant is the same as in wild-type embryos.
TS18: hindlimb bud mesenchyme	Present		4F	Expression in this mutant is the same as in wild-type embryos.
TS18: trunk somite	Present	Regionally restricted	4F	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Absent		4G
TS18: hindlimb bud mesenchyme	Absent		4G
TS18: trunk somite	Trace	Regionally restricted	4G	In these mutants, expression is missing in the ventral tip of thoracic somites and is severely decreased in the dorsal tip.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Absent		4H
TS20: somite	Trace	Regionally restricted	4H	In these mutants, expression is missing in the ventral tip of thoracic somites and is severely decreased in the dorsal tip.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Absent		4I