Probe:	MDK1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	5A	Expression was detected in the head process ectoderm.
TS10: embryo mesoderm	Present		5A

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	5B	Expression detected in the rostral part of the headfolds and subadjacent mesoderm, the caudal regions of the headfolds, the most lateral regions of the mesoderm.
TS11: embryo mesoderm	Present	Regionally restricted	5B	Expression detected in the rostral part of the headfolds and subadjacent mesoderm, the caudal regions of the headfolds, the most lateral regions of the mesoderm.
TS11: future brain	Present	Regionally restricted	5B	Expression detected in the rostral part of the headfolds and subadjacent mesoderm, the caudal regions of the headfolds, the most lateral regions of the mesoderm.
TS11: primitive streak	Absent		5B	Expression was also absent in the mesoderm adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	5C	Expression detected in rostral portion of neural groove neurectoderm and subadjacent mesoderm.
TS11: future brain neural fold	Present	Regionally restricted	5C	Expression detected in rostral portion of neural groove neurectoderm and subadjacent mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	5D	Expression detected in the caudal regions of the headfolds and the most lateral regions of the mesoderm.
TS11: future brain neural fold	Present	Regionally restricted	5D	Expression detected in the caudal regions of the headfolds and the most lateral regions of the mesoderm.
TS11: primitive streak	Absent		5D	Expression was also absent in the mesoderm adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	5E	Expression detected in the caudal regions of the headfolds and the most lateral regions of the mesoderm.
TS11: future brain neural fold	Present	Regionally restricted	5E	Expression detected in the caudal regions of the headfolds and the most lateral regions of the mesoderm.
TS11: primitive streak	Absent		5E	Expression was also absent in the mesoderm adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon neural fold	Strong	Regionally restricted	6A	Expression detected on the edges of the forebrain neural fold,
TS12: head paraxial mesenchyme	Present	Regionally restricted	6A	Expression detected in lateral mesoderm adjacent to 1st somites.
TS12: anterior pro-rhombomere	Present		6A
TS12: posterior pro-rhombomere	Present		6A

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon neural fold	Strong	Regionally restricted	6B	Expression detected on the edges of the forebrain neural fold.
TS12: head paraxial mesenchyme	Present	Regionally restricted	6B	Expression detected in lateral mesoderm adjacent to 1st somites.
TS12: anterior pro-rhombomere	Present		6B
TS12: posterior pro-rhombomere	Present		6B
TS12: head somite	Present	Regionally restricted	6B	Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.
TS12: trunk somite	Present	Regionally restricted	6B	Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 2	Present	Uniform	6C
TS13: rhombomere 5	Present	Uniform	6C
TS13: rhombomere 4	Present	Uniform	6C	Expression in R4 is higher than in R5.
TS13: rhombomere 3	Strong	Uniform	6C
TS13: head somite	Present	Regionally restricted	6C	Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.
TS13: trunk somite	Present	Regionally restricted	6C	Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 2	Strong	Uniform	6D
TS14: rhombomere 3	Strong	Uniform	6D
TS14: rhombomere 5	Strong	Uniform	6D
TS14: rhombomere 4	Present	Uniform	6D
TS14: rhombomere 6	Present	Uniform	6D
TS14: inner ear	Present		6D	Expression detected in the otic vesicle.

Structure	Level	Pattern	Image	Note
TS14: rhombomere 2	Strong	Uniform	6E
TS14: rhombomere 3	Strong	Uniform	6E
TS14: rhombomere 5	Strong	Uniform	6E
TS14: rhombomere 4	Present	Uniform	6E
TS14: rhombomere 6	Present	Uniform	6E
TS14: head somite	Present	Regionally restricted	6E	Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites. Expression detected in the otic vesicle.
TS14: inner ear	Present	Regionally restricted	6E	Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites. Expression detected in the otic vesicle.
TS14: trunk somite	Present	Regionally restricted	6E	Expression was detected in all somites except the two to three caudal pairs that were the most recent to segregate. Expression was restricted to the most dorsal part of the somites. Expression detected in the otic vesicle.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Strong	Uniform	6F
TS13: rhombomere 5	Weak	Uniform	6F
TS13: heart	Absent		6F

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Strong	Uniform	6H	Specimen was double hydridized with Egr2 and Epha7 probes and expression was inferred from previous specimens.
TS13: rhombomere 5	Weak	Uniform	6H	Specimen was double hydridized with Egr2 and Epha7 probes and expression was inferred from previous specimens.

Structure	Level	Pattern	Image	Note
TS15: rhombomere	Strong	Regionally restricted	7A	Signal was maximal along the dorsal edges of the rhombomeres and extends more ventrally in cells of the ventricular layer.

Structure	Level	Pattern	Image	Note
TS15: rhombomere	Strong	Regionally restricted	7B	Signal was maximal along the dorsal edges of the rhombomeres and extends more ventrally in cells of the ventricular layer.
TS15: inner ear	Present		7B

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	7C	Expression is detected in the dorsal part of differentiating somites and the boundary between the dermatome and myotome in rostral somites.

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	7D	Expression is detected in the boundary between the dermatome and myotome in rostral somites.

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present	Regionally restricted	7E	Expression is detected in the dorsal edge of the dermomyotome.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	8B	Expression is detected in two dorsal domains; from the midbrain-forebrain junction to the optic vesicles and at the base of the optic vesicles.
TS13: future prosencephalon	Present	Regionally restricted	8B	Expression is detected in two dorsal domains; from the midbrain-forebrain junction to the optic vesicles and at the base of the optic vesicles.

Structure	Level	Pattern	Image	Note
TS15: rhombomere 2	Strong	Uniform	8C
TS15: rhombomere 3	Strong	Uniform	8C
TS15: rhombomere 5	Strong	Uniform	8C
TS15: rhombomere 4	Present	Uniform	8C
TS15: rhombomere 6	Present	Uniform	8C
TS15: future forebrain	Present	Regionally restricted	8C	Expression is detected at the dorsal midline of the forebrain.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Present	Uniform	9B
TS13: rhombomere 5	Present	Uniform	9B

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Absent		9C
TS13: rhombomere 2	Present	Uniform	9C
TS13: rhombomere 4	Strong	Uniform	9C

Structure	Level	Pattern	Image	Note
TS14: rhombomere 2	Strong	Uniform	9D
TS14: rhombomere 3	Strong	Uniform	9D
TS14: rhombomere 4	Present	Uniform	9D
TS14: rhombomere 5	Present	Uniform	9D

Structure	Level	Pattern	Image	Note
TS14: rhombomere 3	Absent		9E
TS14: rhombomere 2	Present	Uniform	9E
TS14: rhombomere 4	Strong	Uniform	9E

Structure	Level	Pattern	Image	Note
TS14: rhombomere 2	Strong	Uniform	9F
TS14: rhombomere 3	Strong	Uniform	9F
TS14: rhombomere 4	Present	Uniform	9F
TS14: rhombomere 5	Present	Uniform	9F

Structure	Level	Pattern	Image	Note
TS14: rhombomere 3	Absent		9G
TS14: rhombomere 2	Present	Uniform	9G
TS14: rhombomere 4	Present	Uniform	9G

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon neural fold	Present		8A