Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:47294
Thomas T, et al., The bHLH factors, dHAND and eHAND, specify pulmonary and systemic cardiac ventricles independent of left-right sidedness. Dev Biol. 1998 Apr 15;196(2):228-36
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1338199
|
|
Gene symbol:
|
Hand1
|
|
Gene name:
|
heart and neural crest derivatives expressed 1
|
|
Probe:
|
eHAND probe1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
1A E 8.0:
embryonic day 8.0
(more  )
Specimen
1A E 8.0:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
1B E 8.0:
(close )
|
Genetic Background:
|
involves: FVB/N
|
|
Mutant Allele(s):
|
Invsinv/Invsinv
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
|
1B
|
Expression in this mutant is the same as in wild-type embryos.
|
| TS12: lateral plate mesoderm |
Present |
|
1B
|
Expression in this mutant is the same as in wild-type embryos.
|
| TS12: heart |
Present |
Regionally restricted |
1B
|
Expression is interrupted in the anterior and posterior segments of the heart tube. Expression in the mutant is the same as in wild-type embryos.
|
Specimen
1C E 8.5:
embryonic day 8.5
(more )
Specimen
1C E 8.5:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: heart |
Present |
Regionally restricted |
1C
|
Expression is on the left side of the heart tube.
|
Specimen
1D E 8.5:
(close )
|
Genetic Background:
|
involves: FVB/N
|
|
Mutant Allele(s):
|
Invsinv/Invsinv
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: heart |
Present |
Regionally restricted |
1D
|
Expression is on the right side of the heart.
|
Specimen
1E E 10.5:
embryonic day 10.5
(more )
Specimen
1E E 10.5:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart |
Present |
Regionally restricted |
1E
|
Expression restricted to the systemic ventricle. Some expression on the outside edge of the pulmonary ventricle.
|
Specimen
1F E 10.5:
(close )
|
Genetic Background:
|
involves: FVB/N
|
|
Mutant Allele(s):
|
Invsinv/Invsinv
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: heart |
Present |
Regionally restricted |
1F
|
Expression restricted to the systemic ventricle. Some expression on the outside edge of the pulmonary ventricle.
|
Specimen
2B,C:
embryonic day 8.0
(more )
Note: The planes of the sections are shown in FIG. 2A.
Specimen
2B,C:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
The planes of the sections are shown in FIG. 2A.
|
Specimen
3A:
embryonic day 9.5
(more )
Specimen
3A:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
3B:
(close )
|
Genetic Background:
|
involves: FVB/N
|
|
Mutant Allele(s):
|
Invsinv/Invsinv
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
Specimen
4B:
embryonic day 9.5
(more )
Note: The plane of the section is shown in FIG. 4A.
Specimen
4B:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
The plane of the section is shown in FIG. 4A.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: primitive ventricle |
Present |
|
4B
|
|
| TS15: heart |
Present |
Regionally restricted |
4B
|
Although there is expression elsewhere in the heart, there is no expression in the right-side (pulmonary) ventricle.
|
Specimen
4D:
embryonic day 9.5; Invsinv/ Invsinv
(more )
Note: The plane of the section is shown in FIG. 4C.
Specimen
4D:
(close )
|
Genetic Background:
|
involves: FVB/N
|
|
Mutant Allele(s):
|
Invsinv/Invsinv
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
The plane of the section is shown in FIG. 4C.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: primitive ventricle |
Present |
|
4D
|
In this mutant there is still expression in the systemic ventricle.
|
| TS15: heart |
Present |
Regionally restricted |
4D
|
In this mutant there is still no expression in the pulmonary ventricle (although there is expression elsewhere in the heart).
|
|
Analysis Tools