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Gene Expression Data
Assay Details
Assay
Reference: J:41031 Sheth B, et al., Tight junction assembly during mouse blastocyst formation is regulated by late expression of ZO-1 alpha+ isoform. Development. 1997 May;124(10):2027-37
Assay type: RT-PCR
MGI Accession ID: MGI:1338174
Gene symbol: Tjp1
Gene name: tight junction protein 1
Probe: primers-2, primers-4
Assay notes: A two-stage RT-PCR method using primers-1,3 (MGI:1332529) for the first reaction was used.
Results
Image: 2A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 905 bp (a) 665 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
cu P adult TS28: cumulus oophorus Present Present Not Specified Not Specified Female cumulus cells
e P adult TS28: secondary oocyte Absent Present Not Specified Not Specified Female unfertilized eggs
2 E1.0 (c) TS2: 2-cell stage conceptus Absent Present Not Specified Not Specified Not Specified
4 E1.0 (d) TS3: 4-cell stage conceptus Absent Present Not Specified Not Specified Not Specified
pc E2.5 (e) TS3: 8-cell stage conceptus Absent Present Not Specified Not Specified Not Specified
c8 E2.5 (f) TS3: morula-stage conceptus Absent Present Not Specified Not Specified Not Specified
16 E3.0 (g) TS3: morula-stage conceptus Absent Present Not Specified Not Specified Not Specified
lm E3.0 (h) TS4: morula-stage conceptus Present Present Not Specified Not Specified Not Specified
eb E3.5 (i) TS4: blastocyst-stage conceptus Present Present Not Specified Not Specified Not Specified
lb E4.0 (j) TS5: blastocyst-stage conceptus Present Present Not Specified Not Specified Not Specified
c Control
Notes:
(a) This band is only present when the ZO-1alpha+ isoform is expressed.
(b) This band is only present when the ZO-1alpha- isoform is expressed.
(c) 2-cell stage
(d) 4-cell stage
(e) pre-compact 8-cell stage
(f) compact 8-cell stage
(g) 16-cell morula
(h) late morula
(i) early blastocyst
(j) late blastocyst
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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