Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54638
Henderson DJ, et al., Rib truncations and fusions in the Sp2H mouse reveal a role for Pax3 in specification of the ventro-lateral and posterior parts of the somite. Dev Biol. 1999 May 1;209(1):143-58
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1336584
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Gene symbol:
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Myog
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Gene name:
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myogenin
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Probe:
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Myogenin probe7
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Beta-galactosidase
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Results
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Specimen
4.A wt:
embryonic day 10.5
(more  )
Specimen
4.A wt:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4.A Sp2H:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk somite |
Present |
Regionally restricted |
4A right
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Expression domain is truncated ventrally in mutants compared with wild-type. Moreover, positive cells are present outside of the normal expression domain, making the somites appear ragged.
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Specimen
4.B wt:
embryonic day 11.5
(more )
Specimen
4.B wt:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk somite |
Present |
Regionally restricted |
4B left
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Expression domain has extended ventrally into the flank.
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Specimen
4.B Sp2H:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk somite |
Present |
Regionally restricted |
4B right
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Expression domain remains truncated ventrally. Expression domains between individual somites are often fused ventrally in mutant embryos.
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Specimen
4.C:
embryonic day 11.5
(more )
Note: Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
Specimen
4.C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk somite |
Present |
Regionally restricted |
4C
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Expression is detected in the myotome.
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Specimen
4.D:
embryonic day 11.5; Pax3Sp-2H/ Pax3Sp-2H
(more )
Note: Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
Specimen
4.D:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Pax3Sp-2H/Pax3Sp-2H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Embryos were sectioned in 0.45% gelatine, 27% albumin, 18% glucose in PBS which was solidified by the addition of 1/10 volume of 30% glutaraldehyde.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: trunk somite |
Present |
Regionally restricted |
4D
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Myotomal expression domain is truncated ventrally.
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| TS19: trunk sclerotome |
Present |
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4D
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Ectopic expression in seen in the sclerotome in mutants.
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Analysis Tools