Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54643
Storm EE, et al., GDF5 coordinates bone and joint formation during digit development. Dev Biol. 1999 May 1;209(1):11-27
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1336346
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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Gli3 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4D:
embryonic day 12.5
(more  )
Specimen
4D:
(close  )
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Genetic Background:
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BALB/cJ
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb digit |
Present |
Regionally restricted |
4D
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Expression is in the digital ray 30-35 cell diameters distal to the developing metatarsal.
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| TS20: foot |
Present |
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4D
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Specimen
4H:
(close )
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Genetic Background:
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involves: BALB/cJ
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Mutant Allele(s):
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Gdf5bp-3J/Gdf5bp-3J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4H
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Expression becomes restricted to 15-20 cell diameters in the region of the metatarsophalangeal joint.
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Specimen
4L:
(close )
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Genetic Background:
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involves: BALB/cJ
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Mutant Allele(s):
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Gdf5bp-3J/Gdf5bp-3J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4L
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Expression is broad and distal to the metatarsal rather than becoming restricted to the joints as it does in wild-type embryos.
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Specimen
4O:
(close )
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Genetic Background:
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involves: BALB/cJ
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Mutant Allele(s):
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Gdf5bp-3J/Gdf5bp-3J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindlimb digit mesenchyme |
Present |
Regionally restricted |
4O
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Expression becomes restricted to the metatarsophalangeal joints and the 1st and 2nd interphalangeal joints.
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Specimen
4S:
(close )
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Genetic Background:
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involves: BALB/cJ
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Mutant Allele(s):
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Gdf5bp-3J/Gdf5bp-3J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools
