Probe:	Col2a1 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit	Present	Graded	4A	Expression is in the digital rays. Expression is strong in the developing metetarsal region and is less strong distally.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit cartilage condensation	Strong		4E
TS22: hindlimb digit mesenchyme	Weak	Regionally restricted	4E	There is weak expression is in the developing joint.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit cartilage condensation	Strong		4M
TS22: hindlimb digit mesenchyme	Weak	Regionally restricted	4M	Expression is weak in the joint condensation.

Structure	Level	Pattern	Image	Note
TS21: metatarsus pre-cartilage condensation	Strong		4I
TS21: hindlimb phalanx pre-cartilage condensation	Weak		4I	Weaker expression is distal to the metatarsal. This is different than in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS22: hindlimb phalanx cartilage condensation	Present	Graded	4Q	Expression is along the outside of the digital ray in the region of the proximal and medial phalanges, but expression is greatly reduced within the condensation.