Gene Expression Data
Assay Details
Assay
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Reference:
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J:51100
Karl J, et al., Sertoli cells of the mouse testis originate from the coelomic epithelium. Dev Biol. 1998 Nov 15;203(2):323-33
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1336017
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Gene symbol:
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Lama1
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Gene name:
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laminin, alpha 1
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Antibody:
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anti-laminin 1
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Detection system:
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Secondary antibody coupled to fluorescein
|
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Results
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Specimen
11.5 dpc gonads:
embryonic day 11.5
(more )
Specimen
11.5 dpc gonads:
(close )
Genetic Background:
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CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Present |
Regionally restricted |
1A
,
1B
|
Laminin-1 was discontinuously distributed along the basal side of the coelomic epithelium, whereas the basement membrane components are thick and continuous under the mesonephros. Laminin-1 is also deposited on the basal side of the mesonephric tubules.
|
TS19: mesonephric tubule |
Present |
Regionally restricted |
1A
,
1B
|
Laminin-1 was discontinuously distributed along the basal side of the coelomic epithelium, whereas the basement membrane components are thick and continuous under the mesonephros. Laminin-1 is also deposited on the basal side of the mesonephric tubules.
|
Specimen
12.5 dpc testis:
embryonic day 12.5
(more )
Specimen
12.5 dpc testis:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: testis |
Present |
Regionally restricted |
2A
|
Laminin-1 is deposited between the epithelializing Sertoli cells and the peritubular myoid cells as testis cords form. The basement membrane layers under the coelomic epithelium of both the mesonephros and the gonad are thick and continuous.
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|