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Gene Expression Data
Assay Details
Assay
Reference: J:15300 Nieto MA, et al., A receptor protein tyrosine kinase implicated in the segmental patterning of the hindbrain and mesoderm. Development. 1992 Dec;116(4):1137-50
Assay type: RNA in situ
MGI Accession ID: MGI:1335751
Gene symbol: Epha4
Gene name: Eph receptor A4
Probe: probe 2
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: Expression pattern observed was the same when a 5' probe, 3' probe, or kinase domain probe was used.
Results
Specimen 1.C: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: otic placode Strong 1B/C
TS14: rhombomere 3 Strong 1B/C
TS14: rhombomere 5 Strong 1B/C
TS14: rhombomere 2 Moderate 1B/C
TS14: rhombomere 6 Moderate 1B/C
Specimen 2.B: embryonic day 7.75 (more )
Note: 0-somite stage.
Structure Level Pattern Image Note
TS11: future brain Present Regionally restricted 2A/B A broad domain of expression is seen in the hindbrain. Higher levels of expression are coincident with the domain of Egr2 (Krox20) expression; lower levels extend to a boundary in the posterior hindbrain.
TS11: embryo mesoderm Present Graded 2A/B Expression levels were higher in more anterior, mature regions of trunk mesoderm.
Specimen 2.E: embryonic day 8.0 (more )
Note: 4-somite stage.
Structure Level Pattern Image Note
TS12: future brain Present Regionally restricted 2D/E
Specimen 2.H: embryonic day 8.0 (more )
Note: 4-somite stage.
Structure Level Pattern Image Note
TS12: future brain Present Regionally restricted 2G/H This is a higher magnification image of the embryo shown in 2.E.
Specimen 2.K: embryonic day 8.5 (more )
Note: 8-somite stage.
Structure Level Pattern Image Note
TS12: anterior pro-rhombomere Strong Regionally restricted 2J/K Strong expression is detected in the prospective rhombomere 3 and 5 domains while moderate expression is detected in the prospective r2, r4 and r6 domains.
TS12: posterior pro-rhombomere Strong Regionally restricted 2J/K Strong expression is detected in the prospective rhombomere 3 and 5 domains while moderate expression is detected in the prospective r2, r4 and r6 domains.
Specimen 2.N: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: otocyst Strong 2M/N
TS15: rhombomere 3 Strong 2M/N
TS15: rhombomere 5 Strong 2M/N
TS15: rhombomere 2 Moderate 2M/N
TS15: rhombomere 6 Moderate 2M/N
Specimen 3.B: embryonic day 8.5 (more )
Note: 12-somite stage.
Structure Level Pattern Image Note
TS13: rhombomere 3 Strong 3A/B
Specimen 3.E: embryonic day 8.5 (more )
Note: 12-somite stage embryo.
Structure Level Pattern Image Note
TS13: rhombomere 5 Strong 3D/E
TS13: otic placode Present 3D/E
Specimen 4.B: embryonic day 9.5 (more )
Note: 25-somite stage.
Structure Level Pattern Image Note
TS15: rhombomere 3 Present 4A/B
Specimen 4.E: embryonic day 9.5 (more )
Note: 25-somite stage.
Structure Level Pattern Image Note
TS15: rhombomere 3 Present 4D/E
Specimen 4.H: embryonic day 9.5 (more )
Note: 25-somite stage.
Structure Level Pattern Image Note
TS15: rhombomere 5 Present 4G/H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory