Gene Expression Data
Assay Details
Assay
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Reference:
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J:50259
Kil SH, et al., The alpha4 subunit of integrin is important for neural crest cell migration. Dev Biol. 1998 Oct 1;202(1):29-42
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1335747
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Gene symbol:
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Itga4
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Gene name:
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integrin alpha 4
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Antibody:
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PS-2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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Specimen was embedded in gelatin prior to cryosectioning.
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Results
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Specimen
4A:
embryonic day 8.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
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Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
|
Embedding:
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Gelatin
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Specimen
4B:
embryonic day 8.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Specimen
4C:
embryonic day 8.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Specimen
4D:
embryonic day 9.5
(more )
Specimen
4D:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
2% Paraformaldehyde
|
Embedding:
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Gelatin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: trigeminal preganglion |
Strong |
|
4D
|
|
TS15: dorsal root ganglion |
Present |
Regionally restricted |
4D
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In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
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TS15: migrating neural crest |
Present |
Regionally restricted |
4D
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
|
TS15: trunk mesenchyme derived from neural crest |
Present |
Regionally restricted |
4D
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
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Specimen
4E:
embryonic day 9.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: dorsal root ganglion |
Present |
Regionally restricted |
4E
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
|
TS15: migrating neural crest |
Present |
Regionally restricted |
4E
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
|
TS15: trunk mesenchyme derived from neural crest |
Present |
Regionally restricted |
4E
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
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TS15: gut |
Present |
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4E
|
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Specimen
4F:
embryonic day 9.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: dorsal root ganglion |
Present |
Regionally restricted |
4F
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
|
TS15: migrating neural crest |
Present |
Regionally restricted |
4F
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
|
TS15: trunk mesenchyme derived from neural crest |
Present |
Regionally restricted |
4F
|
In the trunk region, neural crest cells displayed integrin alpha-4 immunoreactivity both during their migration as well as during condensation into dorsal root ganglia and other derivatives.
|
TS15: trunk somite |
Present |
Regionally restricted |
4F
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Integrin alpha-4 immunoreactivity was noted in the myotome.
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Specimen
4G:
embryonic day 11.0
(more )
Specimen
4G:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Specimen
4H:
embryonic day 11.0
(more )
Specimen
4H:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Specimen
4I:
embryonic day 11.0
(more )
Specimen
4I:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
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Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Specimen
not shown:
embryonic day 11.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
(C57BL/6 x DBA)F1
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Gelatin
|
|