Probe:	nbl probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present		6A
TS20: central nervous system	Present	Regionally restricted	6A	Expression was detected throughout the neural tube, from rostral telencephalon to caudal spinal cord.
TS20: spinal cord	Present	Regionally restricted	6A	Expression was detected throughout the neural tube, from rostral telencephalon to caudal spinal cord.
TS20: telencephalon	Present	Regionally restricted	6A	Expression was detected throughout the neural tube, from rostral telencephalon to caudal spinal cord.
TS20: cranial ganglion	Present		6A	Expression was detected in all of the cranial sensory ganglia (V to XI).
TS20: facial ganglion	Present		6A	Expression was detected in all of the cranial sensory ganglia (V to XI).
TS20: glossopharyngeal ganglion	Present		6A	Expression was detected in all of the cranial sensory ganglia (V to XI).
TS20: trigeminal ganglion	Present		6A	Expression was detected in all of the cranial sensory ganglia (V to XI).
TS20: vagus ganglion	Present		6A	Expression was detected in all of the cranial sensory ganglia (V to XI).
TS20: vestibulo-cochlear ganglion complex	Present		6A	Expression was detected in all of the cranial sensory ganglia (V to XI).
TS20: olfactory epithelium	Present		6A
TS20: retina	Present		6A

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		6B
TS20: nervous system	Present		6B
TS20: olfactory epithelium	Present		6B
TS20: retina	Present		6B
TS20: spinal cord	Present	Regionally restricted	6B	Expression was detected throughout the neural tube.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		6C
TS20: nervous system	Present		6C
TS20: spinal cord mantle layer	Present	Regionally restricted	6C	In caudal spinal cord, expression was limited to the mantle zone, including the differentiating motor neurons of the ventral horn.

Structure	Level	Pattern	Image	Note
TS20: nervous system	Present		6D
TS20: midbrain mantle layer	Present		6D
TS20: midbrain ventricular layer	Absent		6D

Structure	Level	Pattern	Image	Note
TS20: cortical plate	Present		7A inset