Amh RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:26101 Hacker A, et al., Expression of Sry, the mouse sex determining gene. Development. 1995 Jun;121(6):1603-14
Assay type:	RNase protection
MGI Accession ID:	MGI:1335099
Gene symbol:	Amh
Gene name:	anti-Mullerian hormone

Probe:	Amh probe
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography
Assay notes:	Quantification of these results is shown in Figure 5C. One pair of genital ridges was used in this assay.

Results

	Sample Information		Bands				Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Size Not Specified (c)	Size Not Specified (d)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
B	E3.5	TS4: blastocyst-stage conceptus	Present	Absent	Absent	Absent	Not Specified; total RNA	Parkes		Not Specified	225 blastocysts were used in this lane.
5	E10.5 (e)	TS17: gonad primordium	Present	Absent	Absent	Absent	Not Specified; total RNA	Parkes		Male
8	E10.5 (f)	TS17: gonad primordium	Present	Absent	Absent	Absent	Not Specified; total RNA	Parkes		Male
11/12	E10.5 (g)	TS17: gonad primordium	Present	Absent	Absent	Absent	Not Specified; total RNA	Parkes		Male
14	E11.0 (h)	TS18: gonad primordium	Present	Absent	Absent	Absent	Not Specified; total RNA	Parkes		Male
16	E11.5 (i)	TS19: gonad	Present	Absent	Absent	Weak	Not Specified; total RNA	Parkes		Male
18	E11.5 (j)	TS19: gonad	Present	Absent	Absent	Present	Not Specified; total RNA	Parkes		Male
21	E11.5 (k)	TS19: gonad	Present	Weak	Weak	Present	Not Specified; total RNA	Parkes		Male
24	E12.0 (l)	TS20: testis	Present	Weak	Weak	Present	Not Specified; total RNA	Parkes		Male
27	E12.5 (m)	TS20: testis	Present	Weak	Weak	Present	Not Specified; total RNA	Parkes		Male
32	E12.5 (n)	TS20: testis	Present	Strong	Strong	Very strong	Not Specified; total RNA	Parkes		Male
7pp	P7	TS28: testis	Present	Present	Present	Strong	5 µg; total RNA	Parkes		Male
14pp	P14	TS28: testis	Present	Present	Present	Present	5 µg; total RNA	Parkes		Male
21pp	P21	TS28: testis	Present	Present	Present	Present	5 µg; total RNA	Parkes		Male
28pp	P28	TS28: testis	Present	Present	Present	Present	5 µg; total RNA	Parkes		Male
43pp	P43	TS28: testis	Present	Present	Present	Present	5 µg; total RNA	Parkes		Male
L	P adult	TS28: liver	Present	Absent	Absent	Absent	5 µg; total RNA	Parkes		Male

Notes:
(a) The top band is a protected fragment from a ubiquitously expressed neighboring gene. It serves as a loading control.
(b) The largest protected fragment represents a minor transcription start site.
(c) The middle protected fragment represents a minor transcription start site.
(d) The smallest protected fragment represents the major transcription start site.
(e) 5 somites
(f) 8 somites
(g) 11/12 somites
(h) 14 somites
(i) 16 somites
(j) 18 somites
(k) 21 somites
(l) 24 somites
(m) 27 somites
(n) 32 somites

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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