Gene Expression Data
Assay Details
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Assay
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Reference:
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J:26913
Dickson MC, et al., Defective haematopoiesis and vasculogenesis in transforming growth factor-beta 1 knock out mice. Development. 1995 Jun;121(6):1845-54
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1334716
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Gene symbol:
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Hba-x
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Gene name:
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hemoglobin X, alpha-like embryonic chain in Hba complex
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Probe:
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10.6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The fixation for this assay was 4% paraformaldehyde/0.2% gluteraldehyde.
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Results
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Specimen
3C -/-:
(close  )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J * NIH/Ola
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Mutant Allele(s):
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Tgfb1tm1N/Tgfb1tm1N
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: extraembryonic component |
Present |
Regionally restricted |
3C -/-
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Expression is in red blood cells. There is a lack of positively-expressing cells on the anti-mesometrial side of the yolk sac.
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Specimen
3C +/+:
embryonic day 9.5
(more  )
Specimen
3C +/+:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J * NIH/Ola
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Specimen
3D -/- and 3F:
embryonic day 9.5; Tgfb1tm1N/ Tgfb1tm1N
(more )
Note: These samples are dissected yolk sacs.
Specimen
3D -/- and 3F:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J * NIH/Ola
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Mutant Allele(s):
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Tgfb1tm1N/Tgfb1tm1N
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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These samples are dissected yolk sacs.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: extraembryonic component |
Present |
Regionally restricted |
3F
,
3D -/-
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Expression is in red blood cells. There is a lack of expression on the anti-mesometrial side of the yolk sac.
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Specimen
3D +/+ and 3E:
embryonic day 9.5
(more )
Note: These samples are dissected yolk sacs.
Specimen
3D +/+ and 3E:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J * NIH/Ola
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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These samples are dissected yolk sacs.
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Specimen
Not shown +/+:
embryonic day 8.0
(more )
Specimen
Not shown +/+:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J * NIH/Ola
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: yolk sac |
Present |
Regionally restricted |
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Expression is in red blood cells.
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Specimen
Not shown -/-:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J * NIH/Ola
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Mutant Allele(s):
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Tgfb1tm1N/Tgfb1tm1N
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: yolk sac |
Present |
Regionally restricted |
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Expression in this mutant is the same as in wild-type embryos.
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Analysis Tools