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Gene Expression Data
Assay Details
Assay
Reference: J:19212 Wurst W, et al., Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum. Development. 1994 Jul;120(7):2065-75
Assay type: Immunohistochemistry
MGI Accession ID: MGI:1334709
Gene symbol: En1
Gene name: engrailed 1
Antibody: alpha Enhb-1
Assay notes: The antibody used in this assay cross-reacts with both EN1 and EN2 proteins.
Results
Specimen 4H: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted 4H Expression is at the mid-hindbrain junction.
TS15: future midbrain Present Regionally restricted 4H Expression is at the mid-hindbrain junction.
TS15: future spinal cord Present Regionally restricted 4H Expression is in lateral stripes.
TS15: trunk somite Present Regionally restricted 4H Expression is in lateral stripes.
TS15: forelimb bud ectoderm Present Regionally restricted 4H Expression is in the ventral ectoderm.
TS15: hindlimb ridge ectoderm Present Regionally restricted 4H Expression is in the ventral ectoderm.
Specimen 4I: embryonic day 9.5; En1tm1Alj/En1tm1Alj (more )
Structure Level Pattern Image Note
TS15: forelimb bud ectoderm Absent 4I Expression at the mid-hindbrain junction is assumed to be from EN2 protein.
TS15: future hindbrain Absent 4I Expression at the mid-hindbrain junction is assumed to be from EN2 protein.
TS15: future midbrain Absent 4I Expression at the mid-hindbrain junction is assumed to be from EN2 protein.
TS15: future spinal cord Absent 4I Expression at the mid-hindbrain junction is assumed to be from EN2 protein.
TS15: hindlimb ridge ectoderm Absent 4I Expression at the mid-hindbrain junction is assumed to be from EN2 protein.
TS15: trunk somite Absent 4I Expression at the mid-hindbrain junction is assumed to be from EN2 protein.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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