Gene Expression Data
Assay Details
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Assay
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Reference:
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J:19212
Wurst W, et al., Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum. Development. 1994 Jul;120(7):2065-75
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1334706
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Results
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Specimen
4A:
embryonic day 10.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: nervous system |
Present |
Regionally restricted |
4A
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Expression is restricted to axons. Cranial nerve III is clearly visible and cranial nerve IV is just being formed.
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Specimen
4D:
embryonic day 12.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: nervous system |
Present |
Regionally restricted |
4D
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Expression is exclusively in axons.
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Specimen
4B,C:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: nervous system |
Present |
Regionally restricted |
4B
,
4C
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Expression is exclusively in axons. Cranial nerves III and IV are missing in the mutant.
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Specimen
4E:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: nervous system |
Present |
Regionally restricted |
4E
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Expression is exclusively in axons. Cranial nerves III and IV are missing in mutant embryos. There are also fewer longitudinal axonal projections through the midbrain in mutant embryos.
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Analysis Tools
