Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54177
Gory-Faure S, et al., Role of vascular endothelial-cadherin in vascular morphogenesis. Development. 1999 May;126(10):2093-102
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1334699
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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Cy3
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Assay notes:
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Fixative: 1% paraformaldehyde/0.1 M phosphate buffer pH 7.4.
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Results
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Specimen
5A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5tm1Hub
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: cardiovascular system |
Present |
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5A
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PECAM-1 staining of sections revealed that organization of angiogenic cords into vessels was not fully accomplished.
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Specimen
5B:
embryonic day 9.0; Cdh5tm1Hub/Cdh5 +
(more  )
Specimen
5B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5C & 5E:
embryonic day 9.0; Cdh5tm1Hub/ Cdh5tm1Hub
(more )
Note: Fig. 5E is a higher magnification image.
Specimen
5C & 5E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5tm1Hub
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Fig. 5E is a higher magnification image.
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Specimen
5D & 5F:
embryonic day 9.0; Cdh5tm1Hub/Cdh5 +
(more )
Note: Fig. 5F is a higher magnification image.
Specimen
5D & 5F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Fig. 5F is a higher magnification image.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5tm1Hub
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: extraembryonic vascular system |
Present |
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5G
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Normal establishment of chorioallantoic junction; placenta vascular organization similar to normal mice. Blood islands constituted of blood cells densely packed by a closed endothelial layer. Expression normally located at interendothelial junction.
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Specimen
5H:
embryonic day 9.0; Cdh5tm1Hub/Cdh5 +
(more )
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5tm1Hub
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: yolk sac |
Present |
Regionally restricted |
5I
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Blood islands.
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Specimen
5J:
embryonic day 9.0; Cdh5tm1Hub/Cdh5 +
(more )
Specimen
5J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/cIco
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Mutant Allele(s):
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Cdh5tm1Hub/Cdh5+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: yolk sac |
Present |
Regionally restricted |
5J
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Vitelline vessels of the yolk sac.
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