Probe:	Cryptic cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Single cells	8A	Cryptic is expressed by scattered cells in the mesodermal wings emerging from the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	8A	Cryptic expression begins with localized staining at the anterior end of the primitive streak.
TS10: embryo mesoderm	Present	Spotted	8A	Cryptic is expressed by scattered cells in the mesodermal wings emerging from the primitive streak.

Structure	Level	Pattern	Image	Note
TS11: notochordal process	Present		8B
TS11: embryo	Present	Regionally restricted	8B	Expression becomes confined to a lateral band around the egg cylinder. Expression in a striking 'Easter egg' pattern. Staining is absent from the prechordal plate and paraxial mesoderm. Two symmetrical bands of Cryptic expression extend around the embryo.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	8C	In early and late head-fold stage embryos, Cryptic is expressed in a striking 'Easter egg' pattern. Staining is notably absent from the prechordal plate. Two symmetrical bands of Cryptic expression extend around the embryo.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	8D	In early and late head-fold stage embryos, Cryptic is expressed in a striking 'Easter egg' pattern. Staining is notably absent from the prechordal plate. Two symmetrical bands of Cryptic expression extend around the embryo.
TS11: node	Strong		8D
TS11: notochordal plate	Moderate	Regionally restricted	8D	Staining extends anteriorly along the notochordal plate.
TS11: embryo mesoderm	Present	Regionally restricted	8D	Staining is notably absent from the paraxial mesoderm. Results: late head-fold (embryonic day 7.5; Swiss We

Structure	Level	Pattern	Image	Note
TS11: node	Strong		8E
TS11: notochordal plate	Moderate	Regionally restricted	8E	Staining extends anteriorly along the notochordal plate.
TS11: embryo mesoderm	Present	Regionally restricted	8E	Staining is absent from the paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	8F	In early and late head-fold stage embryos, Cryptic is expressed in a striking 'Easter egg' pattern. Staining is notably absent from the prechordal plate. Two symmetrical bands of Cryptic expression extend around the embryo.
TS11: node	Strong		8F
TS11: notochordal plate	Moderate	Regionally restricted	8F	Staining extends anteriorly along the notochordal plate.
TS11: embryo mesoderm	Present	Regionally restricted	8F	Staining is notably absent from the paraxial mesoderm. Results: late head-fold (embryonic day 7.5; Swiss We

Structure	Level	Pattern	Image	Note
TS12: embryo	Weak	Regionally restricted	8G	Cryptic staining can still be found in the center of the regressing node and in the intermediate mesoderm adjacent to the nascent somites.
TS12: intermediate mesenchyme	Weak	Regionally restricted	8G	Cryptic staining can still be found in the center of the regressing node and in the intermediate mesoderm adjacent to the nascent somites.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	9B , 9E	Cryptic staining is restricted to the ventral mesoderm layer of the node, and is not observed in the overlying dorsal layer that is continuous with the epiblast. Expression is also present in the lateral plate mesoderm.
TS11: node	Present	Regionally restricted	9B , 9E	Cryptic staining is restricted to the ventral mesoderm layer of the node, and is not observed in the overlying dorsal layer that is continuous with the epiblast. Expression is also present in the lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	9F , 9C	In sections that are immediately rostral to the node, staining is in a narrow patch of the adjacent midline neuroectoderm.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	9D , 9G	In frontal sections through more rostral regions of the notochordal plate, this midline neuroectoderm staining becomes slightly broader in extent. Also expression in sections that are immediately rostral to the node.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	9H , 9I	Expression in the prospective floor plate is also observed in transverse sections through the head folds.
TS11: embryo mesoderm	Present	Regionally restricted	9I	Cryptic is expressed by both the somatopleural and splanchnopleural layers of the lateral plate mesoderm, flanking the coelomic cavity. Additionally, Cryptic is specifically expressed by the anterior lateral plate mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	9J	Cryptic is expressed by both the somatopleural and splanchnopleural layers of the lateral plate mesoderm, flanking the coelomic cavity. Additionally, Cryptic is specifically expressed by the anterior lateral plate mesoderm.