Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30819
Wallin J, et al., Pax1 is expressed during development of the thymus epithelium and is required for normal T-cell maturation. Development. 1996 Jan;122(1):23-30
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1333920
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Antibody:
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Anti Pax1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Embryos were fixed in 3% acetic acid in ethanol.
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Results
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Specimen
3a:
embryonic day 10.5
(more  )
Specimen
3a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3b:
embryonic day 12.5
(more )
Specimen
3b:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thymus primordium |
Present |
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3b
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A large proportion of the epithelial cells are positive.
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Specimen
3c:
embryonic day 14.5
(more )
Specimen
3c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thymus primordium |
Present |
Patchy |
3c
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Expression was confined to the cortex and was absent from the medullary region.
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Specimen
3d:
embryonic day 14.5
(more )
Specimen
3d:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
4a:
postnatal week 6
(more )
Specimen
4a:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal week 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: thymus |
Present |
Single cells |
4a
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Expression is in a subpopulation of cortical stromal cells and was absent from the medulla.
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Specimen
4c:
postnatal week 6
(more )
Specimen
4c:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal week 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: thymus |
Present |
Single cells |
4c
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Some subcapsular cells are positive.
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Specimen
4d:
postnatal week 6
(more )
Specimen
4d:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal week 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: thymus |
Present |
Single cells |
4d
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Expression was detected in some reticular cells.
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Specimen
5a,c:
embryonic day 18.5; Pax1un/Pax1 +
(more )
Specimen
5a,c:
(close )
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Genetic Background:
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involves: C3HeB/FeJ * C57BL/6
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Mutant Allele(s):
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Pax1un/Pax1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
5b,d:
(close )
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Genetic Background:
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involves: C3HeB/FeJ * C57BL/6 * CC57BR/MvY
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Mutant Allele(s):
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Pax1Un-s/Pax1un
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: thymus |
Present |
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5b
,
5d
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Expression level was reduced relative to the undulated heterozygous mice.
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Specimen
5e:
(close )
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Genetic Background:
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involves: C57BL/6 * CC57BR/MvY
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Mutant Allele(s):
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Pax1Un-s/Pax1Un-s
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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