Gene Expression Data
Assay Details
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Assay
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Reference:
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J:17224
Bober E, et al., Pax-3 is required for the development of limb muscles: a possible role for the migration of dermomyotomal muscle progenitor cells. Development. 1994 Mar;120(3):603-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1333918
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Gene symbol:
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Myod1
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Gene name:
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myogenic differentiation 1
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Probe:
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MyoD probe4
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Probe preparation:
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labelled with S35 RNA
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Results
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Specimen
control:
embryonic day 11.5
(more  )
Specimen
control:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
Present |
Regionally restricted |
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MyoD was expressed at similar levels in axial and body wall muscles of control and mutant animals.
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| TS19: limb |
Strong |
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Specimen
5F/H:
(close )
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Genetic Background:
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involves: 101/H * C3H * C3H/HeH
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Mutant Allele(s):
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Pax3Sp-1H/Pax3Sp-1H
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: embryo |
Present |
Regionally restricted |
5F/H
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MyoD was expressed at similar levels in axial and body wall muscles of control and mutant animals.
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| TS19: limb |
Absent |
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5F/H
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Specimen
6A:
embryonic day 13.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: vertebral axis musculature |
Present |
Regionally restricted |
6A
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All muscle groups within the limbs, axial and body wall in control animals were highly positive for MyoD.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 101/H * C3H * C3H/HeH
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Mutant Allele(s):
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Pax3Sp-1H/Pax3Sp-1H
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: vertebral axis musculature |
Present |
Regionally restricted |
6B
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No labelling was present in limbs of splotch mutants. MyoD expression was the same in axial and body wall muscles of wild-type and mutant embryos. Occasionally, a slight reduction of deep back muscles was seen at the level of the neural tube defect.
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Analysis Tools
