Probe:	Dlx-2 probe3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present	Regionally restricted	4A	The rostral domain of expression coincided with the medial ganglionic eminence and surrounding structures including the anterior entopeduncular area and posterior preoptic area.
TS22: preoptic area	Present	Regionally restricted	4A	Expression was detected in the posterior preoptic area.
TS22: anterior entopeduncular area	Present		4A
TS22: medial ganglionic eminence	Present		4A
TS22: diencephalon	Present	Regionally restricted	4A , 4B	The caudal domain extended in the alar plate from the area of the suprachiasmatic nucleus through the hypothalamic cell cord and posterior entopeduncular area into the ventral thalamus, or alar p3, and terminated abruptly at the zli.
TS22: hypothalamus	Present	Regionally restricted	4A	Dlx-2 was expressed in the tuberal hypothalamus, with a sharp caudal boundary and a more diffuse rostral boundary.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4C	In the caudal domain, expression appeared normal in alar tissue corresponding to the sch, hcc, pep and in the basal plate in the tuberal hypothalamus, and expression boundaries were similar to those in +/+ embryos, the rostral domain appeared normal.
TS22: hypothalamus	Present	Regionally restricted	4C	In the caudal domain, expression appeared normal in alar tissue corresponding to the sch, hcc, pep and in the basal plate in the tuberal hypothalamus, and expression boundaries were similar to those in +/+ embryos, the rostral domain appeared normal.
TS22: thalamus	Weak	Regionally restricted	4C , 4D	Dlx-2 expression was abnormal in the ventral thalamus. It was very weak and its caudal boundary appeared faded and diffuse in wholemounts.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4E	In the caudal domain, expression appeared normal in alar tissue corresponding to the sch, hcc, pep and in the basal plate in the tuberal hypothalamus, and expression boundaries were similar to those in +/+ embryos, the rostral domain appeared normal.
TS22: hypothalamus	Present	Regionally restricted	4E	In the caudal domain, expression appeared normal in alar tissue corresponding to the sch, hcc, pep and in the basal plate in the tuberal hypothalamus, and expression boundaries were similar to those in +/+ embryos, the rostral domain appeared normal.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Trace	Regionally restricted		Dlx-2 expression was abnormal in the ventral thalamus. Labelling in the ventral thalamus was so weak that it was not seen clearly in sectioned material.