Probe:	Hoxb9 probe2
Assay notes:	The fixative used in this assay was 4% paraformaldehyde/0.1% gluteraldehyde.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	10B wt	Expression was detected in the neural tube posterior to the third somite pair.
TS15: trunk mesenchyme	Present	Regionally restricted	10B wt	Expression was detected in the lateral and somitic mesoderm posterior to the fifth somite pair.
TS15: trunk somite	Present	Regionally restricted	10B wt	Expression was detected in the lateral and somitic mesoderm posterior to the fifth somite pair.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Weak	Regionally restricted	10B -/-	Anterior boundaries of expression were normal, but overall the expression level was reduced compared to wild type.
TS15: trunk mesenchyme	Weak	Regionally restricted	10B -/-	Anterior boundaries of expression were normal, but overall the expression level was reduced compared to wild type.
TS15: trunk somite	Weak	Regionally restricted	10B -/-	Anterior boundaries of expression were normal, but overall the expression level was reduced compared to wild type.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Weak	Regionally restricted	10C	The overall expression level of mutant embryos was reduced compared to wild type. This reduction in expression was most readily apparent in the posteriormost regions of the mutant in the neural folds and lateral mesoderm.
TS13: trunk mesenchyme	Weak	Regionally restricted	10C	The overall expression level of mutant embryos was reduced compared to wild type. This reduction in expression was most readily apparent in the posteriormost regions of the mutant in the neural folds and lateral mesoderm.
TS13: trunk somite	Weak	Regionally restricted	10C	The overall expression level of mutant embryos was reduced compared to wild type. This reduction in expression was most readily apparent in the posteriormost regions of the mutant in the neural folds and lateral mesoderm.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	10D	Expression was detected in the neural tube posterior to the third somite pair level.
TS13: trunk mesenchyme	Present	Regionally restricted	10D	Expression was detected in the lateral and somitic mesoderm, posterior to the level of the fifth somite pair.
TS13: trunk somite	Present	Regionally restricted	10D	Expression was detected in the lateral and somitic mesoderm, posterior to the level of the fifth somite pair.