Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42110
Wassarman KM, et al., Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development. 1997 Aug;124(15):2923-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331429
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Gene symbol:
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Wnt1
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Gene name:
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wingless-type MMTV integration site family, member 1
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Probe:
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Wnt1 probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3E:
embryonic day 10.5
(more  )
Specimen
3E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
3E
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Expression in a transverse band at the posterior end of the midbrain. Expressed in a stripe along the dorsal midline of the midbrain and of the hindbrain posterior to the cerebellar anlage.
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| TS17: midbrain |
Present |
Regionally restricted |
3E
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Expression in a transverse band at the posterior end of the midbrain. Expressed in a stripe along the dorsal midline of the midbrain and of the hindbrain posterior to the cerebellar anlage.
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| TS17: rhombomere 1 |
Absent |
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3E
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| TS17: rhombomere 2 |
Absent |
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3E
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Specimen
3F:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
3F
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Expression in a transverse band at the posterior end of the midbrain. Expressed in a stripe along the dorsal midline of the midbrain and of the hindbrain posterior to the cerebellar anlage.
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| TS17: midbrain |
Present |
Regionally restricted |
3F
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Expression in a transverse band at the posterior end of the midbrain. Expressed in a stripe along the dorsal midline of the midbrain and of the hindbrain posterior to the cerebellar anlage.
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| TS17: rhombomere 1 |
Absent |
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3F
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The non-Wnt1-expression rhombomere1/rhombomere2 is much smaller than in normal embryos.
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| TS17: rhombomere 2 |
Absent |
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3F
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The non-Wnt1-expression rhombomere1/rhombomere2 is much smaller than in normal embryos.
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Specimen
3K:
embryonic day 9.5
(more )
Specimen
3K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3L:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Regionally restricted |
3L
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There are patches of ectopic expression.
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| TS15: rhombomere 1 |
Absent |
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3L
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The non-expressing rhombomere1/rhombomere2 region is severely reduced in size.
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| TS15: rhombomere 2 |
Absent |
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3L
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The non-expressing rhombomere1/rhombomere2 region is severely reduced in size.
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Analysis Tools
