Probe:	Ihh cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6A
TS19: hindlimb bud	Absent		6A

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	6B	Ihh expression was detected in the distal limb bud and tail bud. However, at the posterior margin where Shh was normally expressed, no Ihh expression was detected.
TS19: hindlimb bud	Present	Regionally restricted	6B	Ihh expression was detected in the distal limb bud and tail bud. However, at the posterior margin where Shh was normally expressed, no Ihh expression was detected.
TS19: tail	Present	Regionally restricted	6B	Ihh expression was detected in the distal limb bud and tail bud. However, at the posterior margin where Shh was normally expressed, no Ihh expression was detected.
TS19: branchial arch	Present		6B

Structure	Level	Pattern	Image	Note
TS20: forelimb	Strong	Regionally restricted	6C	Ihh expression is down-regulated in the apical region whereas its expression in the anterior limb bud remained strong.
TS20: hindlimb	Strong	Regionally restricted	6C	Ihh expression is down-regulated in the apical region whereas its expression in the anterior limb bud remained strong.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	6D , 6E	Ectopic Ihh expression in the distal limb bud was quite superficial and was absent in the 'core' mesenchyme.
TS19: hindlimb bud	Present	Regionally restricted	6D , 6E	Ectopic Ihh expression in the distal limb bud was quite superficial and was absent in the 'core' mesenchyme.
TS19: footplate apical ectodermal ridge	Absent		6E	Ectopic Ihh expression was not detected in the distal ectoderm and the AER.
TS19: handplate apical ectodermal ridge	Absent		6E	Ectopic Ihh expression was not detected in the distal ectoderm and the AER.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Weak		7A

Structure	Level	Pattern	Image	Note
TS20: cartilage	Weak		7B

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Weak	Regionally restricted	7C	Ihh is expressed in the cartilage.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present		7D
TS20: embryo	Present	Regionally restricted	7D	Ihh is ectopically expressed in the branchial arches.
TS20: elbow	Present	Regionally restricted	7D	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.
TS20: hand	Present	Regionally restricted	7D	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present		7E
TS20: embryo	Present	Regionally restricted	7E	Ihh is ectopically expressed in the branchial arches.
TS20: elbow	Present	Regionally restricted	7E	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.
TS20: hand	Present	Regionally restricted	7E	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.

Structure	Level	Pattern	Image	Note
TS20: elbow	Present	Regionally restricted	7F	Ihh is ectopically expressed in the dorsal and ventral metacarpal region and future elbow.
TS20: hand	Present	Regionally restricted	7F	Ihh is ectopically expressed in the dorsal and ventral metacarpal region and future elbow.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present		7G
TS20: embryo	Present	Regionally restricted	7G	Ihh is ectopically expressed in the branchial arches.
TS20: elbow	Present	Regionally restricted	7G	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.
TS20: hand	Present	Regionally restricted	7G	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.

Structure	Level	Pattern	Image	Note
TS20: cartilage	Present		7H
TS20: embryo	Present	Regionally restricted	7H	Ihh is ectopically expressed in the branchial arches.
TS20: elbow	Present	Regionally restricted	7H	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.
TS20: hand	Present	Regionally restricted	7H	Ihh is also ectopically expressed in the dorsal and ventral metacarpal region and future elbow.

Structure	Level	Pattern	Image	Note
TS20: elbow	Present	Regionally restricted	7I	Ihh is ectopically expressed in the dorsal and ventral metacarpal region and future elbow.
TS20: hand	Present	Regionally restricted	7I	Ihh is ectopically expressed in the dorsal and ventral metacarpal region and future elbow.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present			No ectopic Ihh expression is detected in mutants.