Probe:	PDGF-R alpha probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	It is possible that PDGF-R[alpha] probe3 (MGI:2448749) was used in this assay.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Strong	Regionally restricted	1B	Expression was detected in clustered mesenchymal cells lining the distal epithelial tubules and terminal buds and was weak surrounding the proximal epithelium of prospective bronchi and branches.

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Strong	Regionally restricted		Expression was detected in clustered mesenchymal cells lining the distal epithelial tubules and terminal buds and was weak surrounding the proximal epithelium of prospective bronchi and branches.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Strong	Regionally restricted		Expression was detected in the mesenchyme surrounding distal epithelial terminal buds. Mutant was indistinguishable from wild type.

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Strong	Regionally restricted	2A	Expression was detected in the mesenchyme surrounding distal epithelial terminal buds.

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Strong	Regionally restricted	2B	Expression was detected in the mesenchyme surrounding distal epithelial terminal buds. Mutant was indistinguishable from wild type.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Strong	Regionally restricted	2C	Expression was detected in the cells at the terminal buds. Cells were more dispersed than at E15.5.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Strong	Regionally restricted	2D	Expression was detected in the distal epithelial terminal buds. In the mutant, there appeared to be fewer cells with no change in their distribution (essentially unchanged from that seen at E15.5) than seen in wild type.

Structure	Level	Pattern	Image	Note
TS25: lung	Present	Single cells	2E	Expression was detected in cells lining the lumen of terminal sacs and was strong in widely scattered numerous cells.
TS25: lung mesenchyme	Weak	Regionally restricted	2E	Expression was detected in the cells surrounding the proximal epithelium and in the prospective bronchial and bronchiolar walls.
TS25: lung vascular element	Weak	Regionally restricted	2E	Expression was detected in the walls.

Structure	Level	Pattern	Image	Note
TS25: lung	Present	Regionally restricted	2F	Expression was strong in clustered cells at the proximal tubule and was detected in thin walled tubules of the prospective terminal sacs but in hardly any cells compared to wild type.

Structure	Level	Pattern	Image	Note
TS25: lung	Present	Single cells	2G	Expression was detected in cells lining the lumen of terminal sacs and was strong in widely scattered numerous cells.
TS25: lung mesenchyme	Weak	Regionally restricted	2G	Expression was detected in the cells surrounding the proximal epithelium and in the prospective bronchial and bronchiolar walls.
TS25: lung vascular element	Weak	Regionally restricted	2G	Expression was detected in the walls.

Structure	Level	Pattern	Image	Note
TS25: lung	Present	Regionally restricted	2H	Expression was strong in clustered cells at the proximal tubule and was detected in thin walled tubules of the prospective terminal sacs but in hardly any cells compared to wild type.

Structure	Level	Pattern	Image	Note
TS26: lung	Present	Single cells		Expression was detected in numerous cells in the walls of the terminal sacs.

Structure	Level	Pattern	Image	Note
TS26: lung	Absent			There were no or very few positive cells in the walls of the terminal sacs.

Structure	Level	Pattern	Image	Note
TS27: lung vascular element	Present		2I	Expression was detected in the vascular walls.
TS27: alveolar wall	Strong		2I	Strong expression in numerous cells in the walls of the terminal sacs.

Structure	Level	Pattern	Image	Note
TS27: lung vascular element	Present		2J	Expression was detected in the vascular walls.
TS27: alveolar wall	Trace		2J	Few or no positive cells in the walls of the terminal sacs.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present			Expression was detected in the vascular walls.
TS28: alveolar wall	Present			Expression in numerous cells in the walls of the terminal sacs, but the number of cells declined from that seen at P1.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present			Expression was detected in the vascular walls.
TS28: alveolar sac	Trace			Few or no positive cells in the walls of the terminal sacs.

Structure	Level	Pattern	Image	Note
TS28: alveolar wall	Present		2K	Expression was detected in numerous cells in the walls of the terminal sacs, but the number of cells declined from that seen at P1.

Structure	Level	Pattern	Image	Note
TS28: alveolar wall	Absent		2L	Expression was absent from cells in the walls of the terminal sacs.

Structure	Level	Pattern	Image	Note
TS28: lung connective tissue	Present	Regionally restricted	4C	Expression was detected in scattered mesenchymal cells.
TS28: alveolar septum	Present	Single cells	4C	Only a few cells were labeled from the alveolar septa.

Structure	Level	Pattern	Image	Note
TS28: lung connective tissue	Present	Regionally restricted	4D	Expression was detected in mesenchymal cells.
TS28: alveolar septum	Present	Single cells	4D	Only a few cells were labeled from the alveolar septa.

Structure	Level	Pattern	Image	Note
TS28: alveolus smooth muscle	Absent			Expression was absent from the alveolar smooth muscle cells.