Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43763
Gavalas A, et al., Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning. Development. 1997 Oct;124(19):3693-702
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1330754
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Gene symbol:
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Nkx1-2
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Gene name:
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NK1 homeobox 2
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Probe:
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Sax-1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1G:
embryonic day 10.5
(more  )
Note: Also stained for Hoxb1 (assay MGI:1330755).
Specimen
1G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for Hoxb1 (assay MGI:1330755).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 |
Present |
Regionally restricted |
1G
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Expression is in the ventral portion of rhombomere 1 with a sharp caudal boundary at rhombomere 2.
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| TS17: rhombomere 1 floor plate |
Present |
Regionally restricted |
1G
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A narrow stripe of cells next to the floorplate extends caudally.
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| TS17: rhombomere 2 floor plate |
Present |
Regionally restricted |
1G
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A narrow stripe of cells next to the floorplate extends caudally.
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Specimen
1H:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hoxa2tm1Ipc/Hoxa2tm1Ipc
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for Hoxb1 (assay MGI:1330755).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 1 |
Present |
Regionally restricted |
1H
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The sharp rhombomere1/2 boundary does not exist in these mutants; expression expands posteriorly into rhombomere 2.
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| TS17: rhombomere 2 |
Present |
Regionally restricted |
1H
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The sharp rhombomere1/2 boundary does not exist in these mutants; expression expands posteriorly into rhombomere 2.
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| TS17: rhombomere 3 |
Ambiguous |
Regionally restricted |
1H
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Expression may extend into rhombomere 3 in these mutants.
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Analysis Tools