Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50087
Davis TL, et al., Imprinted expression and methylation of the mouse H19 gene are conserved in extraembryonic lineages. Dev Genet. 1998;23(2):111-8
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:1330732
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Gene symbol:
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H19
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Gene name:
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H19, imprinted maternally expressed transcript
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Probe:
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H19-pI, H19-pJ
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Probe preparation:
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labelled with P32 DNA
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Visualized with:
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Autoradiography
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Assay notes:
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To discriminate between alleles, PCR products were run on denaturing poly-acrylamide gels and subjected to single-strand conformation polymorphism (SSCP) analysis.
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Results
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Image: 1C
| P w 3
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TS28: musculature
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Present
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Not Applicable
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Not Specified |
C57BL/6J |
| Not Specified |
| P w 3
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TS28: musculature
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Absent
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Present
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Not Specified |
(C57BL/6J x CAST-H19cBy)F1 |
| Not Specified |
| E8.5
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TS13: embryo
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Present
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Absent
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Not Specified |
(C57BL/6J x CAST-H19cBy)F1 |
| Not Specified |
| E9.5
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TS15: embryo
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Present
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Absent
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Not Specified |
(C57BL/6J x CAST-H19cBy)F1 |
| Not Specified |
| E10.5
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TS17: embryo
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Present
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Absent
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Not Specified |
(C57BL/6J x CAST-H19cBy)F1 |
| Not Specified |
| E11.5
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TS19: embryo
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Present
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Absent
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Not Specified |
(C57BL/6J x CAST-H19cBy)F1 |
| Not Specified |
| E12.5
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TS20: embryo
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Present
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Absent
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Not Specified |
(C57BL/6J x CAST-H19cBy)F1 |
| Not Specified |
Notes:
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Analysis Tools
