Eif4e RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:50182 De Sousa PA, et al., Transient expression of a translation initiation factor is conservatively associated with embryonic gene activation in murine and bovine embryos. Biol Reprod. 1998 Oct;59(4):969-77
Assay type:	RT-PCR
MGI Accession ID:	MGI:1329603
Gene symbol:	Eif4e
Gene name:	eukaryotic translation initiation factor 4E

Probe:	Eif4e-pA, Eif4e-pB
Probe preparation:	DNA
Visualized with:	Ethidium bromide

Results

Image: 2_eIF-4E

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	525 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
GV	P adult (a)	TS28: oocyte	Present (b)	Not Specified; total RNA	CF-1		Female
O	P adult (c)	TS28: oocyte	Present (d)	Not Specified; total RNA	CF-1		Female
1c	E0.5	TS1: 1-cell stage conceptus	Present (e)	Not Specified; total RNA	CF-1		Not Specified
2c	E1.5	TS2: 2-cell stage conceptus	Present (e)	Not Specified; total RNA	CF-1		Not Specified
4c	E2.0 (f)	TS3: 4-cell stage conceptus	Present (g)	Not Specified; total RNA	CF-1		Not Specified
M	E2.5	TS3: morula-stage conceptus	Present (h)	Not Specified; total RNA	CF-1		Not Specified
B	E3.5 (i)	TS4: blastocyst-stage conceptus	Present (j)	Not Specified; total RNA	CF-1		Not Specified

Notes:
(a) Germinal vesicle-intact oocytes
(b) signal=100%
(c) Metaphase II-arrested oocytes.
(d) signal=20%
(e) signal=40%
(f) 4-cell embryo
(g) signal=30%
(h) signal=35%
(i) blastocyst
(j) signal=60%

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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