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Gene Expression Data
Assay Details
Assay
Reference: J:50182 De Sousa PA, et al., Transient expression of a translation initiation factor is conservatively associated with embryonic gene activation in murine and bovine embryos. Biol Reprod. 1998 Oct;59(4):969-77
Assay type: RT-PCR
MGI Accession ID: MGI:1329601
Gene symbol: Eif5
Gene name: eukaryotic translation initiation factor 5
Probe: Eif5-pA, Eif5-pB
Probe preparation: DNA
Visualized with: Ethidium bromide
Results Image: 2_eIF-5
 Sample Information Bands Other Sample Information
Lane AgeStructure 367 bp Amount Genetic BackgroundMutant Allele(s)Sex
GV P adult (a) TS28: oocyte Present (b) Not Specified; total RNA CF-1 Female
O P adult (c) TS28: oocyte Present (d) Not Specified; total RNA CF-1 Female
1c E0.5 TS1: 1-cell stage conceptus Present (e) Not Specified; total RNA CF-1 Not Specified
2c E1.5 TS2: 2-cell stage conceptus Present (f) Not Specified; total RNA CF-1 Not Specified
4c E2.0 (g) TS3: 4-cell stage conceptus Present (h) Not Specified; total RNA CF-1 Not Specified
M E2.5 TS3: morula-stage conceptus Present (i) Not Specified; total RNA CF-1 Not Specified
B E3.5 (j) TS4: blastocyst-stage conceptus Present (k) Not Specified; total RNA CF-1 Not Specified
Notes:
(a) Germinal vesicle-intact oocytes
(b) signal=100%
(c) Metaphase II-arrested oocytes.
(d) signal=25%
(e) signal=45%
(f) signal=40%
(g) 4-cell embryo
(h) signal=60%
(i) signal=70%
(j) blastocyst
(k) signal=120%
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory