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Gene Expression Data
Assay Details
Assay
Reference: J:49839 Zhu Y, et al., Smad3 mutant mice develop metastatic colorectal cancer. Cell. 1998 Sep 18;94(6):703-14
Assay type: RT-PCR
MGI Accession ID: MGI:1329585
Gene symbol: Smad3
Gene name: SMAD family member 3
Probe: P1, P8
Probe preparation: DNA
Visualized with: Ethidium bromide
Assay notes: Panel A P1/8, Primers amplify the entire gene.
Results Image: 3A P1/8
 Sample Information Bands Other Sample Information
Lane AgeStructure 1.3 kb (b) 1 kb (c) Amount Genetic BackgroundMutant Allele(s)Sex
+/+ E14.5 (a) TS22: embryo Present Not Applicable Not Specified; total RNA Not Specified Not Specified
+/- E14.5 (a) TS22: embryo Present Present Not Specified; total RNA either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Smad3tm1Par/Smad3+Not Specified
-/- E14.5 (a) TS22: embryo Absent Present Not Specified; total RNA either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) Smad3tm1Par/Smad3tm1ParNot Specified
Ctr Control
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) wild-type transcript
(c) mutant transcript

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory