Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51129
Bierkamp C, et al., Desmosomal localization of beta-catenin in the skin of plakoglobin null-mutant mice. Development. 1999 Jan;126(2):371-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1328153
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Gene symbol:
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Dsc1
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Gene name:
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desmocollin 1
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Antibody:
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anti-Desmocollins
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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The antibody did not discriminate between related genes, Dsc2 (MGI:103221) and Dsc3 (MGI:1194993) have also been annotated.
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Results
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Specimen
Not Shown:
embryonic day 12.5
(more  )
Specimen
Not Shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: skin |
Present |
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Expression was present in desmosomes.
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Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: skin |
Present |
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No differences were observed between mutant and wild type staining.
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Specimen
Not Shown:
embryonic day 15.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: skin |
Strong |
Regionally restricted |
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Expression was strong in the suprabasal layer. Expression was present in desmosomes.
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Specimen
Not Shown:
(close )
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Genetic Background:
|
either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: skin |
Strong |
Regionally restricted |
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Expression was strong in the suprabasal layer. No differences were observed between mutant and wild type staining.
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Specimen
Not Shown:
embryonic day 17.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Spotted |
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Expression was present in desmosomes.
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Specimen
Not Shown:
(close )
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Genetic Background:
|
either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Regionally restricted |
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Expression was diffusely distributed over the cell membrane and in the cytoplasm.
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