Antibody:	anti-SOX1 pAb
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS11: future spinal cord neural plate	Present		1A
TS11: embryo	Present	Regionally restricted	1A	Expression of SOX1 is detected at 7.5 dpc in the anterior half of the embryo.

Structure	Level	Pattern	Image	Note
TS12: anterior pro-rhombomere neural fold	Present	Uniform	1B	SOX1 is maintained in all neuroepithelial cells along the entire anteroposterior axis of the neural folds.
TS12: future hindbrain neural fold	Present	Uniform	1B	SOX1 is maintained in all neuroepithelial cells along the entire anteroposterior axis of the neural folds.
TS12: future midbrain neural fold	Present	Uniform	1B	SOX1 is maintained in all neuroepithelial cells along the entire anteroposterior axis of the neural folds.
TS12: future prosencephalon neural fold	Present	Uniform	1B	SOX1 is maintained in all neuroepithelial cells along the entire anteroposterior axis of the neural folds.
TS12: future spinal cord neural fold	Present	Uniform	1B	SOX1 is maintained in all neuroepithelial cells along the entire anteroposterior axis of the neural folds.
TS12: posterior pro-rhombomere neural fold	Present	Uniform	1B	SOX1 is maintained in all neuroepithelial cells along the entire anteroposterior axis of the neural folds.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		1C

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	1D	SOX1 labelling is detected in the early neural tube but is excluded from the ventral midline region of the floor plate.
TS14: neural tube floor plate	Present	Regionally restricted	1D	SOX1 labelling is detected in the early neural tube but is excluded from the ventral midline region of the floor plate.

Structure	Level	Pattern	Image	Note
TS16: future spinal cord	Present	Regionally restricted	1F , 1E	SOX1 labelling is not detected in the ventral motor horns. SOX1 is maintained in 'region X', bilateral streams of cells between the floor plate and motor horns.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Regionally restricted		By 13.5 dpc, SOX1 expression is restricted to a thin ventricular zone in the CNS.
TS21: peripheral nervous system	Absent

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2A	SOX1 expression is detected throughout the pseudostratified epithelium of the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2C	SOX1 expression is detected throughout the pseudostratified epithelium of the posterior neural tube.

Structure	Level	Pattern	Image	Note
TS17: midbrain ventricular layer	Present		2G , 2E	SOX1 expression is restricted to the ventricular zone in the more mature anterior region of the neural tube.