Probe:	Tlx2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS8: embryo	Absent		1a

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		1b
TS10: embryo	Present		1b	Expression was observed in the proximal lateral region.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Weak	Regionally restricted		Weak staining was also observed in the anterior ectoderm after longer colour development.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	1c	Expression was observed in the ectoderm.
TS10: embryo ectoderm	Present	Regionally restricted	1c	Expression was observed in the ectoderm of anterior proximal region.
TS10: extraembryonic ectoderm	Present		1c
TS10: embryo endoderm	Absent		1c
TS10: embryo mesoderm	Absent		1c

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	1d	Expression was seen in the extreme posterior end of the primitive streak.
TS11: embryo	Present	Regionally restricted	1d	Expressed in two symmetrical stripes that extended laterally on either side of the streak, corresponding to the lateral part of the neuroepithelium.
TS11: node	Absent		1d

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	1e	Expressed in both the ectoderm and mesoderm at the posterior end of the primitive streak at this level.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	1f	Expression in the stripes occurred in the prospective neural ectoderm and was excluded from the midline and the mesoderm.

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	1g	Prominent expression in the posterior end of the primitive streak.
TS12: node	Weak		1g
TS12: embryo	Present	Regionally restricted	1g	Expressed in two symmetrical stripes that extended laterally on either side of the streak.