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Gene Expression Data
Assay Details
Assay
Reference: J:49322 Zuniga A, et al., Gli3 (Xt) and formin (ld) participate in the positioning of the polarising region and control of posterior limb-bud identity. Development. 1999 Jan;126(1):13-21
Assay type: RNA in situ
MGI Accession ID: MGI:1314737
Gene symbol: Gli1
Gene name: GLI-Kruppel family member GLI1
Probe: Gli cDNA3
Probe preparation: labelled with digoxigenin RNA
Assay notes: All results were confirmed by also analyzing ld<ln2>/Xt<H> intercrosses.
Results
Specimen 6e: embryonic day 11.0; Gli3Xt-J/Gli3Xt-J (more )
Note: ~40 somites.
Structure Level Pattern Image Note
TS18: hindlimb bud Strong Graded 6e Gli expression is highest in cells close to the Shh domain.

Specimen 6f: embryonic day 11.0; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: ~40 somites.
Structure Level Pattern Image Note
TS18: hindlimb bud Strong Graded 6f Gli expression is highest in cells close to the Shh domain.

Specimen 6g: embryonic day 11.0; Gli3Xt-J/Gli3Xt-J, Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: ~40 somites.
Structure Level Pattern Image Note
TS18: hindlimb bud Present Graded 6g Xt;ld double homozygous hindlimb bud with posterior Gli expression. Gli expression is highest in cells close to the Shh domain.

Specimen 6h: embryonic day 11.0; Gli3Xt-J/Gli3Xt-J, Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: ~40 somites.
Structure Level Pattern Image Note
TS18: hindlimb bud Present Graded 6h Xt;ld double homozygous hindlimb bud with apical Gli expression. Gli expression is highest in cells close to the Shh domain.

Specimen not shown: embryonic day 11.0 (more )
Note: ~40 somites.
Structure Level Pattern Image Note
TS18: hindlimb bud Strong


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory